Human Fibrinogen Like Protein 1 (FGL1) ELISA Kit | FGL1 elisa kit

Human Fibrinogen Like Protein 1 (FGL1) ELISA Kit

Cat. Num. AAA2704873

• Gene Names: FGL1; HFREP1; HP-041; LFIRE1; LFIRE-1
• Reactivity: Human
• Synonyms: Fibrinogen Like Protein 1 (FGL1); Human Fibrinogen Like Protein 1 (FGL1) ELISA Kit; FGL-1; HFREP1; HP-041; LFIRE1; Hepassocin; Hepatocyte-derived fibrinogen-related protein 1; Liver fibrinogen-related protein 1; FGL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibrinogen Like Protein 1 (FGL1).; No significant cross-reactivity or interference between Fibrinogen Like Protein 1 (FGL1) and analogues was observed.
• Sequence Length: 312

• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.074ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Like Protein 1 (FGL1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Like Protein 1 (FGL1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGL1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Like Protein 1 (FGL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Like Protein 1 (FGL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Like Protein 1 (FGL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibrinogen Like Protein 1 (FGL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 42544202
• NCBI GeneID: 2267
• NCBI Accession #: NP_963847.1
• NCBI GenBank Nucleotide #: NM_201553.1
• UniProt Accession #: Q08830; Q4PJH9; Q53YF1; Q8NG32; Q96KW6; Q96QM6; A6NKU4
• Molecular Weight: 36,379 Da
• NCBI Official Full Name: fibrinogen-like protein 1
• NCBI Official Synonym Full Names: fibrinogen like 1
• NCBI Official Symbol: FGL1
• NCBI Official Synonym Symbols: HFREP1; HP-041; LFIRE1; LFIRE-1
• NCBI Protein Information: fibrinogen-like protein 1
• UniProt Protein Name: Fibrinogen-like protein 1
• Protein Family: Fibrinogen-like protein
• UniProt Gene Name: FGL1
• UniProt Synonym Gene Names: HFREP1; HFREP-1; LFIRE-1

NCBI Description

Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Has hepatocyte mitogenic activity.