Typical Testing Data/Standard Curve (for reference only)
Human Fecal Calprotectin ELISA Kit
Fecal Calprotectin, Human, Rapid ELISA kit
Reactivity
Human
Synonyms
Fecal Calprotectin; Human; Rapid ELISA kit; S100A8/S100A9; MRP8/MRP14; Fecal Calprotectin Human Rapid ELISA kit 1 x 96 det.; Fecal Calprotectin elisa kit
Reactivity
Human
Samples
Feces
Preparation and Storage
Product should be stored at 4 degree C.
Under recommended storage conditions, product is stable for at least six months.
Under recommended storage conditions, product is stable for at least six months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Fecal Calprotectin elisa kit
Intended Uses: The fecal Calprotectin ELISA kit is to be used for the in vitro quantitative determination of human calprotectin in feces. This kit is intended for laboratory research use only. The analysis should be performed by trained laboratory professionals.
Background/Introduction: Calprotectin, also known as S100A8/A9 heterocomplex, is formed out of the calcium-binding, migrating proteins S100A8 and S100A9. Calprotectin is a calcium- and zinc-binding protein complex composed of 8 and 14 kD subunits and complex formation is calcium dependent. Neutrophils are the main producers of calprotectin, but expression has also been found in monocytes, macrophages and ileal tissue eosinophils. Altogether, S100A8 and S100A9 proteins contribute to approximately 40-60% of the soluble, cytosolic content of granulocytes. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Elevated concentrations of calprotectin can be measured among others in plasma, synovial fluid, urine and feces. The presence of calprotectin in feces quantitatively relates to neutrophil migration towards the gastrointestinal tract. Calprotectin is therefore considered a reliable biomarker for intestinal inflammation throughout the gastrointestinal tract. Calprotectin in feces is stable for several days and even longer at 4 degree C. Consistently higher fecal calprotectin levels have been reported in both adult and pediatric patients with IBD compared to IBS or healthy controls. Raised levels of the protein indicate presence of luminal white cells. Such finding indicates that the intestine is inflamed, although the type of inflammation is not defined. The ELISA is a single incubation step reaction. After this incubation the wells are filled with substrate. The obtained color intensity is proportional to the amount of calprotectin in your sample. The amount of calprotectin can be calculated based on the standard curve of calibrated native calprotectin. The test performance is assured by the addition of a high control and a low control.
Background/Introduction: Calprotectin, also known as S100A8/A9 heterocomplex, is formed out of the calcium-binding, migrating proteins S100A8 and S100A9. Calprotectin is a calcium- and zinc-binding protein complex composed of 8 and 14 kD subunits and complex formation is calcium dependent. Neutrophils are the main producers of calprotectin, but expression has also been found in monocytes, macrophages and ileal tissue eosinophils. Altogether, S100A8 and S100A9 proteins contribute to approximately 40-60% of the soluble, cytosolic content of granulocytes. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Elevated concentrations of calprotectin can be measured among others in plasma, synovial fluid, urine and feces. The presence of calprotectin in feces quantitatively relates to neutrophil migration towards the gastrointestinal tract. Calprotectin is therefore considered a reliable biomarker for intestinal inflammation throughout the gastrointestinal tract. Calprotectin in feces is stable for several days and even longer at 4 degree C. Consistently higher fecal calprotectin levels have been reported in both adult and pediatric patients with IBD compared to IBS or healthy controls. Raised levels of the protein indicate presence of luminal white cells. Such finding indicates that the intestine is inflamed, although the type of inflammation is not defined. The ELISA is a single incubation step reaction. After this incubation the wells are filled with substrate. The obtained color intensity is proportional to the amount of calprotectin in your sample. The amount of calprotectin can be calculated based on the standard curve of calibrated native calprotectin. The test performance is assured by the addition of a high control and a low control.
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Product Notes
The Human Fecal Calprotectin (Catalog #AAA584845) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA584845 ELISA Kit recognizes Human Fecal Calprotectin. It is sometimes possible for the material contained within the vial of "Fecal Calprotectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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