Human Fas Apoptotic Inhibitory Molecule 3 (FAIM3) ELISA Kit | FAIM3 elisa kit

Human Fas Apoptotic Inhibitory Molecule 3 (FAIM3) ELISA Kit

Cat. Num. AAA451281

• Gene Names: FCMR; TOSO; FAIM3
• Reactivity: Human
• Synonyms: Fas Apoptotic Inhibitory Molecule 3 (FAIM3); Human Fas Apoptotic Inhibitory Molecule 3 (FAIM3) ELISA Kit; TOSO; FCMR; Regulator of Fas-induced apoptosis Toso; FAIM3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FAIM3. No significant cross-reactivity or interference between FAIM3 and analogues was observed.
• Sequence Length: 278

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FAIM3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FAIM3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FAIM3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FAIM3 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FAIM3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FAIM3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FAIM3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FAIM3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 216547554
• NCBI GeneID: 9214
• NCBI Accession #: NP_001135945.1
• NCBI GenBank Nucleotide #: NM_001142473.1
• UniProt Accession #: O60667; A8K7J2; B7Z6Z0; D9MWM3
• Molecular Weight: 33,828 Da
• NCBI Official Full Name: fas apoptotic inhibitory molecule 3 isoform b
• NCBI Official Synonym Full Names: Fc fragment of IgM receptor
• NCBI Official Symbol: FCMR
• NCBI Official Synonym Symbols: TOSO; FAIM3
• NCBI Protein Information: fas apoptotic inhibitory molecule 3
• UniProt Protein Name: Fas apoptotic inhibitory molecule 3
• UniProt Gene Name: FCMR

NCBI Description

Fc receptors specifically bind to the Fc region of immunoglobulins (Igs) to mediate the unique functions of each Ig class. FAIM3 encodes an Fc receptor for IgM (see MIM 147020) (Kubagawa et al., 2009 [PubMed 19858324]; Shima et al., 2010 [PubMed 20042454]).[supplied by OMIM, Jul 2010]

Uniprot Description

May play a role in the immune system processes. Protects cells from FAS-, TNF alpha- and FADD-induced apoptosis without increasing expression of the inhibitors of apoptosis BCL2 and BCLXL. Seems to activate an inhibitory pathway that prevents CASP8 activation following FAS stimulation, rather than blocking apoptotic signals downstream. May inhibit FAS-induced apoptosis by preventing CASP8 processing through CFLAR up-regulation.