Rabbit anti-Human FNTA Polyclonal Antibody | anti-FNTA antibody

FNTA, NT (FNTA, Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha, CAAX farnesyltransferase subunit alpha, FTase-alpha, Ras proteins prenyltransferase subunit alpha, Type I protein geranyl-geranyltransferase subunit alpha) (PE)

Cat. Num. AAA6299565

• Gene Names: FNTA; FPTA; PTAR2; PGGT1A
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FNTA; Polyclonal Antibody; NT (FNTA; Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha; CAAX farnesyltransferase subunit alpha; FTase-alpha; Ras proteins prenyltransferase subunit alpha; Type I protein geranyl-geranyltransferase subunit alpha) (PE); Type I protein geranyl-geranyltransferase subunit alpha; anti-FNTA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-FNTA antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FNTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-79 amino acids from the N-terminal region of human FNTA.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FNTA (arrow) using rabbit polyclonal FNTA Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FNTA gene.)

Related Product Information for anti-FNTA antibody

Prenyltransferases can attach either a farnesyl group or a geranylgeranyl group in thioether linkage to the cysteine residue of proteins with a C-terminal CAAX box. CAAX geranylgeranyltransferase and CAAX farnesyltransferase are heterodimers that share the same alpha subunit but have different beta subunits. This gene encodes the alpha subunit of these transferases. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 11 and 13.

Product Categories/Family for anti-FNTA antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Lipkin, S.M., et al. Cancer Prev Res (Phila Pa) 3(5):597-603(2010). Fontaine-Bisson, B., et al. J. Mol. Med. 88(2):193-201(2010). Zhou, J., et al. J. Biol. Chem. 284(15):9648-9655(2009). Veluthakal, R., et al. Diabetes 56(1):204-210(2007). Armstrong, S.A., et al. J. Biol. Chem. 270(14):7864-7868(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4503771
• NCBI GeneID: 2339
• NCBI Accession #: NP_002018.1
• NCBI GenBank Nucleotide #: NM_002027.2
• UniProt Accession #: P49354; Q53XJ9; Q9UDC1; A6NJW0; AAB26814
• Molecular Weight: 36,493 Da
• NCBI Official Full Name: protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
• NCBI Official Synonym Full Names: farnesyltransferase, CAAX box, alpha
• NCBI Official Symbol: FNTA
• NCBI Official Synonym Symbols: FPTA; PTAR2; PGGT1A
• NCBI Protein Information: protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha; FTase-alpha; GGTase-I-alpha; farnesyl-protein transferase alpha-subunit; ras proteins prenyltransferase subunit alpha; type I protein geranyl-geranyltransferase alpha subunit; pro
• UniProt Protein Name: Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
• Protein Family: Protein farnesyltransferase/geranylgeranyltransferase
• UniProt Gene Name: FNTA
• UniProt Synonym Gene Names: GGTase-I-alpha
• UniProt Entry Name: FNTA_HUMAN

NCBI Description

Prenyltransferases can attach either a farnesyl group or a geranylgeranyl group in thioether linkage to the cysteine residue of proteins with a C-terminal CAAX box. CAAX geranylgeranyltransferase and CAAX farnesyltransferase are heterodimers that share the same alpha subunit but have different beta subunits. This gene encodes the alpha subunit of these transferases. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq, May 2010]

Uniprot Description

FNTA: Catalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha subunit is thought to participate in a stable complex with the substrate. The beta subunit binds the peptide substrate. Through RAC1 prenylation and activation may positively regulate neuromuscular junction development downstream of MUSK. Belongs to the protein prenyltransferase subunit alpha family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.5.1.59; EC 2.5.1.58

Chromosomal Location of Human Ortholog: 8p11

Cellular Component: microtubule associated complex; cytoplasm; cytosol

Molecular Function: protein binding; protein geranylgeranyltransferase activity; microtubule binding; CAAX-protein geranylgeranyltransferase activity; acetylcholine receptor regulator activity; receptor tyrosine kinase binding; alpha-tubulin binding; protein farnesyltransferase activity; Rab-protein geranylgeranyltransferase activity

Biological Process: protein geranylgeranylation; rhodopsin mediated signaling; phototransduction, visible light; protein farnesylation; regulation of rhodopsin mediated signaling; apoptosis; neurotransmitter receptor metabolic process; transforming growth factor beta receptor signaling pathway; regulation of receptor activity; cell structure disassembly during apoptosis