Human Family With Sequence Similarity 132, Member A (FAM132A) ELISA Kit | FAM132A elisa kit

Human Family With Sequence Similarity 132, Member A (FAM132A) ELISA Kit

Cat. Num. AAA450236

• Gene Names: Fam132a; C1qdc2; CTRP12; alipolin; 1110035L05Rik
• Reactivity: Human
• Synonyms: Family With Sequence Similarity 132; Member A (FAM132A); Human Family With Sequence Similarity 132; Member A (FAM132A) ELISA Kit; C1QDC2; C1QTNF12; Adipolin; Adipose-derived insulin-sensitizing factor; Complement C1q tumor necrosis factor-related protein 12; FAM132A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FAM132A. No significant cross-reactivity or interference between FAM132A and analogues was observed.
• Sequence Length: 308

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FAM132A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FAM132A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FAM132A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FAM132A in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FAM132A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FAM132A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FAM132A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FAM132A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13385632
• NCBI GeneID: 67389
• NCBI Accession #: NP_080401.1
• NCBI GenBank Nucleotide #: NM_026125.3
• UniProt Accession #: Q8R2Z0; Q9CQI8
• Molecular Weight: 33,267 Da
• NCBI Official Full Name: adipolin
• NCBI Official Synonym Full Names: family with sequence similarity 132, member A
• NCBI Official Symbol: Fam132a
• NCBI Official Synonym Symbols: C1qdc2; CTRP12; alipolin; 1110035L05Rik
• NCBI Protein Information: adipolin
• UniProt Protein Name: Adipolin
• Protein Family: Adipolin
• UniProt Gene Name: Fam132a
• UniProt Synonym Gene Names: C1qdc2; C1qtnf12; Ctrp12

Uniprot Description

Insulin-sensitizing adipocyte-secreted protein (adipokine) that regulates glucose metabolism in liver and adipose tissue. Promotes glucose uptake in adipocytes and suppresses de novo glucose production in hepatocytes via the PI3K-Akt signaling pathway. Administration lead to reduction of blood glucose. Able to attenuate inflammation in fat tissue (PubMed:21849507, PubMed:22275362).