Typical Testing Data/Standard Curve (for reference only)
Human Family With Sequence Similarity 132, Member A (FAM132A) ELISA Kit | FAM132A elisa kit
Human Family With Sequence Similarity 132, Member A (FAM132A) ELISA Kit
Gene Names
FAM132A; C1QDC2; CTRP12; C1QTNF12
Reactivity
Human
Synonyms
Family With Sequence Similarity 132; Member A (FAM132A); Human Family With Sequence Similarity 132; Member A (FAM132A) ELISA Kit; C1QDC2; C1QTNF12; Adipolin; Adipose-derived insulin-sensitizing factor; Complement C1q tumor necrosis factor-related protein 12; FAM132A elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of FAM132A. No significant cross-reactivity or interference between FAM132A and analogues was observed.
Sequence Length
302
Samples
tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
<0.054ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FAM132A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FAM132A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FAM132A elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FAM132A in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FAM132A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FAM132A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FAM132A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FAM132A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FAM132A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FAM132A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FAM132A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FAM132A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FAM132A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
32,416 Da
NCBI Official Full Name
Family with sequence similarity 132, member A
NCBI Official Synonym Full Names
family with sequence similarity 132, member A
NCBI Official Symbol
FAM132A
NCBI Official Synonym Symbols
C1QDC2; CTRP12; C1QTNF12
NCBI Protein Information
adipolin
UniProt Protein Name
Adipolin
Protein Family
UniProt Gene Name
FAM132A
UniProt Synonym Gene Names
C1QDC2; CTRP12
UniProt Entry Name
ADIPL_HUMAN
Uniprot Description
FAM132A: Belongs to the FAM132 family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1p36.33
Cellular Component: extracellular space; extracellular region
Molecular Function: hormone activity
Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of glucose import; negative regulation of inflammatory response; negative regulation of gluconeogenesis; regulation of glucose import; positive regulation of insulin receptor signaling pathway
Research Articles on FAM132A
Similar Products
Product Notes
The Human FAM132A fam132a (Catalog #AAA2019571) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2019571 ELISA Kit recognizes Human FAM132A. It is sometimes possible for the material contained within the vial of "Family With Sequence Similarity 132, Member A (FAM132A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
