Human Factor VIII ELISA Kit | F8 elisa kit

Cyno monkey Factor VIII total antigen assay ELISA kit

Cat. Num. AAA136015

• Gene Names: F8; AHF; F8B; F8C; HEMA; FVIII; DXS1253E
• Reactivity: Human
• Synonyms: Factor VIII; Cyno monkey Factor VIII total antigen assay ELISA kit; F8 elisa kit

• Reactivity: Human
• Sequence Length: 2351

• Shipping Note: This product cannot be shipped outside of USA.
• Preparation and Storage: Store at 4 degree C

Related Product Information for F8 elisa kit

Factor VIII (aka Factor VIII:C or Antihemophilic Globulin) is a glycoprotein zymogen that circulates in a stabilized non-covalent complex with von Willebrand Factor (vWF). Following activation by thrombin or Factor Xa, Factor VIIIa dissociates from vWF and catalyzes the activation of Factor X by Factor IXa in the amplification phase of coagulation. Factor VIIIa activity is quickly decreased by spontaneous dissociation and proteolytic degradation by activated Protein C, Factor Xa and Factor IXa. Hemophilia A is caused by mutations in the Factor VIII gene; a majority of patients have decreased Factor VIII plasma levels while 5% of patients have normal levels of nonfunctioning protein. The sensitive quantitative measurement of total cynomolgus macaque monkey (Macaca fascicularis) Factor VIII antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. The average normal plasma level of Factor VIII is defined as 1.0 IU/ml and the normal range is 0.4-1.8 IU/ml. Hemaophilia A patients are classified by the following Factor VIII levels: 0.05-0.25 IU/ml = mild, 0.01-0.05 IU/ml = moderate, and less than 0.01 IU/ml = severe. Normal values of Factor VIII in cyno plasma have not been conclusively determined but are believed to be similar to human plasma. The assay measures total cyno Factor VIII in the 0.003-1.42 IU/ml range. Samples giving cyno Factor VIII levels above 1.42 IU/ml should be diluted in blocking buffer before use. 1:8 and 1:16 dilutions for normal plasma, or 1:4 and 1:8 dilutions for Haemophiliac plasma, are suggested for best results
Cyno Factor VIII will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-cyno Factor VIII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of cyno plasma. Color development is proportional to the concentration of Factor VIII in the samples. All reagents and standards are provided in these ELISA kits.

Product Categories/Family for F8 elisa kit

CLOTTING FACTOR ELISA Kits; Cyno Monkey Factor VIII ELISA Kits; Monkey Kits

NCBI and Uniprot Product Information

• NCBI GI #: 182803
• NCBI GeneID: 2157
• Molecular Weight: 24,641 Da
• NCBI Official Full Name: factor VIII
• NCBI Official Synonym Full Names: coagulation factor VIII
• NCBI Official Symbol: F8
• NCBI Official Synonym Symbols: AHF; F8B; F8C; HEMA; FVIII; DXS1253E
• NCBI Protein Information: coagulation factor VIII
• UniProt Protein Name: Coagulation factor VIII
• Protein Family: Factor VIII intron 22 protein
• UniProt Gene Name: F8
• UniProt Synonym Gene Names: F8C; AHF
• UniProt Entry Name: FA8_HUMAN

Uniprot Description

F8: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Defects in F8 are the cause of hemophilia A (HEMA). A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non- functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: endoplasmic reticulum lumen; ER to Golgi transport vesicle; ER-Golgi intermediate compartment membrane; extracellular region; plasma membrane

Molecular Function: protein binding

Biological Process: blood coagulation; blood coagulation, intrinsic pathway; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; platelet degranulation

Disease: Hemophilia A