Human Exonuclease 1 (EXO1) ELISA Kit | EXO1 elisa kit

Human Exonuclease 1 (EXO1)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2125268

• Gene Names: EXO1; HEX1; hExoI
• Reactivity: Human
• Applications: ELISA
• Synonyms: Exonuclease 1 (EXO1); Human Exonuclease 1 (EXO1)(Sandwich ELISA) ELISA Kit; ELISA Kit for Exonuclease 1 (EXO1); EXO1/Exonuclease 1; HEX1; hExoI; EXO1 elisa kit

• Reactivity: Human
• Specificity: Reactive with Human Exonuclease 1 (EXO1); This assay has high sensitivity and excellent specificity for detection of Exonuclease 1 (EXO1). No significant cross-reactivity or interference between Exonuclease 1 (EXO1) and analogues was observed.

• Applicable Applications for EXO1 elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Detection Method: Double-antibody Sandwich
• Assay Length: 3 hours
• Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Exonuclease 1 (EXO1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Exonuclease 1 (EXO1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100.; Intra-Assay: CV<10%.; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Dehydrogenase 1 (GLUD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Dehydrogenase 1 (GLUD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Dehydrogenase 1 (GLUD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutamate Dehydrogenase 1 (GLUD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Preparation and Storage: Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for EXO1 elisa kit

Enzyme & Kinase; Endocrinology; Neuro science

NCBI and Uniprot Product Information

• NCBI GI #: 284055276
• NCBI GeneID: 9156
• NCBI Accession #: NP_003677.4
• NCBI GenBank Nucleotide #: NM_003686.4
• UniProt Accession #: Q9UQ84; O60545; O75214; O75466; Q5T396; Q96IJ1; Q9UG38; Q9UNW0
• Molecular Weight: 94,103 Da
• NCBI Official Full Name: exonuclease 1 isoform a
• NCBI Official Synonym Full Names: exonuclease 1
• NCBI Official Symbol: EXO1
• NCBI Official Synonym Symbols: HEX1; hExoI
• NCBI Protein Information: exonuclease 1; hExo1; rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
• UniProt Protein Name: Exonuclease 1
• Protein Family: Exonuclease
• UniProt Gene Name: EXO1
• UniProt Synonym Gene Names: EXOI; HEX1; hExo1; hExoI
• UniProt Entry Name: EXO1_HUMAN

NCBI Description

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

EXO1: 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: nucleoplasm; nucleus

Molecular Function: 5'-3' exonuclease activity; protein binding; structure-specific DNA binding; double-stranded DNA specific 5'-3' exodeoxyribonuclease activity; DNA binding; single-stranded DNA specific 5'-3' exodeoxyribonuclease activity; 5'-3' exodeoxyribonuclease activity; metal ion binding; exonuclease activity; ribonuclease H activity; flap endonuclease activity

Biological Process: humoral immune response mediated by circulating immunoglobulin; mismatch repair; meiotic cell cycle; somatic hypermutation of immunoglobulin genes; isotype switching; DNA repair; DNA catabolic process, exonucleolytic; DNA catabolic process, endonucleolytic; DNA recombination