Human Epigen (EPG) ELISA Kit | EPG elisa kit

Human Epigen (EPG) ELISA Kit

Cat. Num. AAA2019223

• Gene Names: EPGN; EPG; PRO9904; ALGV3072
• Reactivity: Human
• Synonyms: Epigen (EPG); Human Epigen (EPG) ELISA Kit; EPGN; Epithelial Mitogen Homolog; EPG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Epigen (EPG).; No significant cross-reactivity or interference between Epigen (EPG) and analogues was observed.
• Sequence Length: 154

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epigen (EPG) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epigen (EPG) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPG elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epigen (EPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epigen (EPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epigen (EPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epigen (EPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for EPG elisa kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 401782583
• NCBI GeneID: 255324
• NCBI Accession #: NP_001257918.1
• NCBI GenBank Nucleotide #: NM_001270989.1
• UniProt Accession #: Q6UW88; A1BMM3; A1BMM4; A1BMM5; A1BMM6; A1BMM7; A1BMM8; A8K090
• Molecular Weight: 8,149 Da
• NCBI Official Full Name: epigen isoform 1
• NCBI Official Synonym Full Names: epithelial mitogen
• NCBI Official Symbol: EPGN
• NCBI Official Synonym Symbols: EPG; PRO9904; ALGV3072
• NCBI Protein Information: epigen
• UniProt Protein Name: Epigen
• UniProt Gene Name: EPGN
• UniProt Synonym Gene Names: EPG
• UniProt Entry Name: EPGN_HUMAN

NCBI Description

The protein encoded by this gene is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. This protein has been reported to have high mitogenic activity but low affinity for its receptor. Expression of this transcript and protein have been reported in cancer specimens of the breast, bladder, and prostate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

Epigen: Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; integral to plasma membrane

Molecular Function: growth factor activity; epidermal growth factor receptor binding

Biological Process: positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; positive regulation of cell proliferation; MAPKKK cascade; angiogenesis; positive regulation of epithelial cell proliferation