Human Epidermal Growth Factor (EGF) ELISA Kit | EGF elisa kit

Human Epidermal Growth Factor (EGF) ELISA Kit

Cat. Num. AAA454595

• Gene Names: EGF; URG; HOMG4
• Reactivity: Human
• Synonyms: Epidermal Growth Factor (EGF); Human Epidermal Growth Factor (EGF) ELISA Kit; URG Beta-Urogastrone Pro-epidermal growth factor; EGF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EGF. No significant cross-reactivity or interference between EGF and analogues was observed.
• Sequence Length: 1166

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 3.906-250pg/mL
• Sensitivity: 1.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EGF elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EGF in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 296011013
• NCBI GeneID: 1950
• NCBI Accession #: NP_001171601.1
• NCBI GenBank Nucleotide #: NM_001178130.2
• UniProt Accession #: P01133; Q52LZ6; B4DRK7; E7EVD2; E9PBF0
• Molecular Weight: 129,746 Da
• NCBI Official Full Name: pro-epidermal growth factor isoform 2
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: Pro-epidermal growth factor
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).