Human Ephrin-B3 (EFNB3) ELISA Kit | EFNB3 elisa kit

Human Ephrin-B3 (EFNB3) ELISA Kit

Cat. Num. AAA287698

• Gene Names: EFNB3; EFL6; EPLG8; LERK8
• Reactivity: Human
• Synonyms: Ephrin-B3 (EFNB3); Human Ephrin-B3 (EFNB3) ELISA Kit; EFL6; EPLG8; LERK8; Ephrin B3; eph-related receptor tyrosine kinase ligand 8; EFNB3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human EFNB3. No significant cross-reactivity or interference between Human EFNB3 and analogues was observed.
• Sequence Length: 340

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for EFNB3 elisa kit

This assay employs a two-site sandwich ELISA to quantitate EFNB3 in samples. An antibody specific for EFNB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFNB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFNB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503489
• NCBI GeneID: 1949
• NCBI Accession #: NP_001397.1
• NCBI GenBank Nucleotide #: NM_001406.3
• UniProt Accession #: Q15768; O00680; Q8TBH7; Q92875; B2RBW2; D3DTQ6
• Molecular Weight: 35,835 Da
• NCBI Official Full Name: ephrin-B3
• NCBI Official Synonym Full Names: ephrin B3
• NCBI Official Symbol: EFNB3
• NCBI Official Synonym Symbols: EFL6; EPLG8; LERK8
• NCBI Protein Information: ephrin-B3
• UniProt Protein Name: Ephrin-B3
• Protein Family: Ephrin
• UniProt Gene Name: EFNB3
• UniProt Synonym Gene Names: EPLG8; LERK8; LERK-8

NCBI Description

EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons ().