Human Ephrin A2 (EFNA2) ELISA Kit | EFNA2 elisa kit

Human Ephrin A2 (EFNA2) ELISA Kit

Cat. Num. AAA2705363

• Gene Names: EFNA2; ELF-1; EPLG6; LERK6; HEK7-L; LERK-6
• Reactivity: Human
• Synonyms: Ephrin A2 (EFNA2); Human Ephrin A2 (EFNA2) ELISA Kit; ELF-1; EPLG6; HEK7-L; LERK6; EPH-related receptor tyrosine kinase ligand 6; HEK7 ligand; EFNA2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ephrin A2 (EFNA2).; No significant cross-reactivity or interference between Ephrin A2 (EFNA2) and analogues was observed.
• Sequence Length: 213

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin A2 (EFNA2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin A2 (EFNA2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EFNA2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin A2 (EFNA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin A2 (EFNA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin A2 (EFNA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin A2 (EFNA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27894381
• NCBI GeneID: 1943
• NCBI Accession #: NP_001396.2
• NCBI GenBank Nucleotide #: NM_001405.3
• UniProt Accession #: O43921; O76020
• Molecular Weight: 23,878 Da
• NCBI Official Full Name: ephrin-A2
• NCBI Official Synonym Full Names: ephrin A2
• NCBI Official Symbol: EFNA2
• NCBI Official Synonym Symbols: ELF-1; EPLG6; LERK6; HEK7-L; LERK-6
• NCBI Protein Information: ephrin-A2
• UniProt Protein Name: Ephrin-A2
• Protein Family: Ephrin
• UniProt Gene Name: EFNA2
• UniProt Synonym Gene Names: EPLG6; LERK6; LERK-6; HEK7-L

NCBI Description

This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNA2: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. With the EPHA2 receptor may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. Belongs to the ephrin family.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: neuromuscular junction; perikaryon; plasma membrane

Molecular Function: ephrin receptor binding

Biological Process: axon guidance; bone remodeling; cell-cell signaling; ephrin receptor signaling pathway; olfactory bulb development; osteoclast differentiation