Human Enkephalin (ENK) ELISA Kit | ENK elisa kit
Human Enkephalin (ENK) ELISA Kit
Gene Names
Penk; Enk; Penk1; Penk2; Penk-rs
Reactivity
Human
Synonyms
Enkephalin (ENK); Human Enkephalin (ENK) ELISA Kit; ENK elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ENK. No significant cross-reactivity or interference between ENK and analogues was observed.
Sequence Length
269
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Competitive inhibition
Detection Range
15.62-1000pg/mL
Sensitivity
6.85pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ENK were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ENK were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Related Product Information for ENK elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ENK in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ENK has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ENK and unlabeled ENK (Standards or samples) with the pre-coated antibody specific to ENK. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ENK in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ENK in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ENK has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ENK and unlabeled ENK (Standards or samples) with the pre-coated antibody specific to ENK. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ENK in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ENK in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,932 Da
NCBI Official Full Name
enkephalin
NCBI Official Synonym Full Names
proenkephalin
NCBI Official Symbol
Penk
NCBI Official Synonym Symbols
Enk; Penk1; Penk2; Penk-rs
NCBI Protein Information
proenkephalin-A
UniProt Protein Name
Proenkephalin-A
UniProt Gene Name
Penk
UniProt Synonym Gene Names
Penk-rs; Penk1; OGF
NCBI Description
neuropeptide hormone; plays a role in the responses to stress and pain perception [RGD, Feb 2006]
Uniprot Description
PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family.
Protein type: Hormone; Secreted; Secreted, signal peptide
Research Articles on ENK
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Product Notes
The Human ENK penk (Catalog #AAA455641) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA455641 ELISA Kit recognizes Human ENK. It is sometimes possible for the material contained within the vial of "Enkephalin (ENK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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