Typical Standard Curve
Rat ENK (enkephalin) ELISA Kit | ENK elisa kit
Rat ENK (enkephalin)
Gene Names
Penk; Enk; Penk1; Penk2; Penk-rs
Reactivity
Rat
Synonyms
ENK (enkephalin); Rat ENK (enkephalin); ENK elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of ENK . No significant cross-reactivity or interference between ENK and analogues was observed.
Sequence Length
269
Samples
Serum, plasma and other biological fluids.
Assay Type
Competitive ELISA, Coated with Antigen
Detection Range
0.625-40ng/ml
Sensitivity
0.375ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ENK were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ENK were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
4 degree C for 6 months
Typical Standard Curve
Typical Standard Curve
Related Product Information for ENK elisa kit
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with ENK . During the reaction,ENK in the sample or standard competes with a fixed amount of ENK on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to ENK . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ENK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,932 Da
NCBI Official Full Name
enkephalin
NCBI Official Synonym Full Names
proenkephalin
NCBI Official Symbol
Penk
NCBI Official Synonym Symbols
Enk; Penk1; Penk2; Penk-rs
NCBI Protein Information
proenkephalin-A
UniProt Protein Name
Proenkephalin-A
UniProt Gene Name
Penk
UniProt Synonym Gene Names
Penk-rs; Penk1; OGF
UniProt Entry Name
PENK_RAT
NCBI Description
neuropeptide hormone; plays a role in the responses to stress and pain perception [RGD, Feb 2006]
Uniprot Description
PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family.
Protein type: Secreted, signal peptide; Secreted; Hormone
Research Articles on ENK
Similar Products
Product Notes
The Rat ENK penk (Catalog #AAA766210) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA766210 ELISA Kit recognizes Rat ENK. It is sometimes possible for the material contained within the vial of "ENK (enkephalin), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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