Human ELA3B ELISA Kit | ELA3B elisa kit

Human ELA3B (Elastase 3B) ELISA Kit

Cat. Num. AAA2514081

• Gene Names: CELA3B; E1; CBPP; EL-1; ELA3B
• Reactivity: Human
• Synonyms: ELA3B; Human ELA3B (Elastase 3B) ELISA Kit; ELA3B elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human ELA3B. No significant cross-reactivity or interference between Human ELA3B and analogues was observed.
• Sequence Length: 270

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ELA3B concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELA3B elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ELA3B. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ELA3B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ELA3B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ELA3B. You can calculate the concentration of ELA3B in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13528831
• NCBI GeneID: 23436
• UniProt Accession #: P08861; P11423; Q5VU28; Q5VU29; Q5VU30; B2RE44
• Molecular Weight: 29,263 Da
• NCBI Official Full Name: ELA3B protein
• NCBI Official Synonym Full Names: chymotrypsin-like elastase family, member 3B
• NCBI Official Symbol: CELA3B
• NCBI Official Synonym Symbols: E1; CBPP; EL-1; ELA3B
• NCBI Protein Information: chymotrypsin-like elastase family member 3B; cholesterol-binding pancreatic protease; elastase 1; elastase 3B, pancreatic; elastase IIIB; elastase-3B; fecal elastase 1; pancreatic elastase 1; pancreatic endopeptidase E; protease E; proteinase E
• UniProt Protein Name: Chymotrypsin-like elastase family member 3B
• UniProt Gene Name: CELA3B
• UniProt Synonym Gene Names: ELA3B
• UniProt Entry Name: CEL3B_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009]

Uniprot Description

CELA3B: Efficient protease with alanine specificity but only little elastolytic activity. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Protease; EC 3.4.21.70

Chromosomal Location of Human Ortholog: 1p36.12

Molecular Function: peptidase activity; serine-type endopeptidase activity

Biological Process: cholesterol metabolic process; proteolysis