Human E3 ubiquitin-protein ligase UBR1 ELISA Kit | UBR1 elisa kit

Human E3 ubiquitin-protein ligase UBR1 ELISA Kit

Cat. Num. AAA766007

• Gene Names: UBR1; JBS
• Reactivity: Human
• Synonyms: E3 ubiquitin-protein ligase UBR1; Human E3 ubiquitin-protein ligase UBR1 ELISA Kit; UBR1/Ubiquitin-protein ligase E3-alpha-I/N-recognin-1/E3 ubiquitin-protein ligase UBR1/N-recognin-1; UBR1 elisa kit

• Reactivity: Human
• Sequence Length: 1749

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for UBR1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human UBR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human UBR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human UBR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman UBR1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 28372497
• NCBI GeneID: 197131
• NCBI Accession #: NP_777576.1
• NCBI GenBank Nucleotide #: NM_174916.2
• Molecular Weight: 93,184 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase UBR1
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3 component n-recognin 1
• NCBI Official Symbol: UBR1
• NCBI Official Synonym Symbols: JBS
• NCBI Protein Information: E3 ubiquitin-protein ligase UBR1
• UniProt Protein Name: E3 ubiquitin-protein ligase UBR1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: UBR1
• UniProt Entry Name: UBR1_HUMAN

NCBI Description

The N-end rule pathway is one proteolytic pathway of the ubiquitin system. The recognition component of this pathway, encoded by this gene, binds to a destabilizing N-terminal residue of a substrate protein and participates in the formation of a substrate-linked multiubiquitin chain. This leads to the eventual degradation of the substrate protein. The protein described in this record has a RING-type zinc finger and a UBR-type zinc finger. Mutations in this gene have been associated with Johanson-Blizzard syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

UBR1: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. Defects in UBR1 are a cause of Johanson-Blizzard syndrome (JBS). This disorder includes congenital exocrine pancreatic insufficiency, multiple malformations such as nasal wing aplasia, and frequent mental retardation. Pancreas of individuals with JBS do not express UBR1 and show intrauterine- onset destructive pancreatitis. Belongs to the UBR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ligase; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 15q13

Cellular Component: cytoplasm; cytosol; proteasome complex; ubiquitin ligase complex

Molecular Function: ligase activity; zinc ion binding

Biological Process: negative regulation of TOR signaling pathway; protein ubiquitination

Disease: Johanson-blizzard Syndrome