Human DNase-I ELISA Kit | DNase-I elisa kit

Human DNase-I (Deoxyribonuclease I) ELISA Kit

Cat. Num. AAA2515385

• Reactivity: Human
• Synonyms: DNase-I; Human DNase-I (Deoxyribonuclease I) ELISA Kit; DNase-I elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human DNase- I in samples. No significant cross-reactivity or interference between Human DNase- I and analogues was observed.
• Sequence Length: 282

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.16-10ng/mL
• Sensitivity: 0.1ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human DNase- I were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human DNase- I were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DNase-I elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human DNase- I concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DNase- I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DNase- I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DNase- I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human DNase- I. You can calculate the concentration of Human DNase- I in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 181624
• Molecular Weight: 19,771 Da
• NCBI Official Full Name: DNase I
• UniProt Protein Name: Deoxyribonuclease-1
• UniProt Gene Name: DNASE1
• UniProt Synonym Gene Names: DNL1; DRNI; DNase I
• UniProt Entry Name: DNAS1_HUMAN

Uniprot Description

DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family.

Protein type: Motility/polarity/chemotaxis; EC 3.1.21.1; Secreted, signal peptide; Deoxyribonuclease; Secreted

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular region; nuclear envelope; nucleus

Molecular Function: protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity

Biological Process: apoptosis; DNA catabolic process, endonucleolytic

Disease: Systemic Lupus Erythematosus