Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) Standard Curve.)
Human Disintegrin And Metalloproteinase Domain-Containing Protein 28 (ADAM28) ELISA Kit | ADAM28 elisa kit
Human Disintegrin And Metalloproteinase Domain-Containing Protein 28 (ADAM28) ELISA Kit
Gene Names
ADAM28; MDCL; MDC-L; eMDCII; ADAM 28; eMDC II
Reactivity
Human
Synonyms
Disintegrin And Metalloproteinase Domain-Containing Protein 28 (ADAM28); Human Disintegrin And Metalloproteinase Domain-Containing Protein 28 (ADAM28) ELISA Kit; ADAM28; ADAM23; MDC-Lm; MDC-Ls; MDCL; eMDCII; a disintegrin and metalloproteinase domain 28; metalloproteinase-like; disintegrin-like; and cysteine-rich protein-L; ADAM28 elisa kit
Reactivity
Human
Specificity
Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM28. No significant cross-reactivity or interference between Human ADAM28 and analogues was observed.
Sequence Length
754
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) Standard Curve.)
Related Product Information for ADAM28 elisa kit
Description: This Human Disintegrin and metalloproteinase domain-containing protein 28 (ADAM28) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM28 in samples. An antibody specific for ADAM28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM28 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ADAM28 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.
Background: ADAM28 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 28 isoform 4 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 28
NCBI Official Symbol
ADAM28
NCBI Official Synonym Symbols
MDCL; MDC-L; eMDCII; ADAM 28; eMDC II
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 28
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 28
Protein Family
UniProt Gene Name
ADAM28
UniProt Synonym Gene Names
ADAM23; MDCL; ADAM 28; eMDC II; MDC-L
NCBI Description
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Uniprot Description
ADAM28: May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.24.-; Membrane protein, integral; Motility/polarity/chemotaxis; Protease
Chromosomal Location of Human Ortholog: 8p21.2
Molecular Function: metallopeptidase activity
Biological Process: spermatogenesis
Research Articles on ADAM28
Similar Products
Product Notes
The Human ADAM28 adam28 (Catalog #AAA9714579) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714579 ELISA Kit recognizes Human ADAM28. It is sometimes possible for the material contained within the vial of "Disintegrin And Metalloproteinase Domain-Containing Protein 28 (ADAM28), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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