Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data

Rat Dickkopf Related Protein 1 ELISA Kit | DKK1 elisa kit

Rat Dickkopf Related Protein 1 ELISA Kit

Gene Names
DKK1; SK; DKK-1
Reactivity
Rat
Synonyms
Dickkopf Related Protein 1; Rat Dickkopf Related Protein 1 ELISA Kit; DKK1/dickkopf (Xenopus laevis) homolog 1/dickkopf homolog 1 (Xenopus laevis)/dickkopf related protein-1/Dickkopf-1/dickkopf-related protein 1/Dkk-1/DKK-1/hDkk-1/SKdickkopf-1 like; DKK1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Sequence Length
266
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for DKK1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat DKK1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat DKK1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat DKK1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat DKK1 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,672 Da
NCBI Official Full Name
dickkopf-related protein 1
NCBI Official Synonym Full Names
dickkopf WNT signaling pathway inhibitor 1
NCBI Official Symbol
DKK1
NCBI Official Synonym Symbols
SK; DKK-1
NCBI Protein Information
dickkopf-related protein 1
UniProt Protein Name
Dickkopf-related protein 1
Protein Family
UniProt Gene Name
DKK1
UniProt Synonym Gene Names
Dickkopf-1; Dkk-1; hDkk-1
UniProt Entry Name
DKK1_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]

Uniprot Description

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: early endosome membrane; extracellular region; extracellular space; plasma membrane

Molecular Function: growth factor activity; low-density lipoprotein receptor binding; protein binding; receptor antagonist activity; signal transducer activity

Biological Process: cellular morphogenesis during differentiation; embryonic limb morphogenesis; endoderm formation; forebrain development; hair follicle development; mesoderm formation; negative regulation of BMP signaling pathway; negative regulation of mesodermal cell fate specification; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein binding; negative regulation of skeletal muscle development; negative regulation of synaptogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway; regulation of endodermal cell fate specification; regulation of receptor internalization; response to retinoic acid

Research Articles on DKK1

Similar Products

Product Notes

The Rat DKK1 dkk1 (Catalog #AAA765467) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA765467 ELISA Kit recognizes Rat DKK1. It is sometimes possible for the material contained within the vial of "Dickkopf Related Protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.