Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Destrin (DSTN) Standard Curve.)
Human Destrin (DSTN) ELISA Kit | DSTN elisa kit
Human Destrin (DSTN) ELISA Kit
Gene Names
DSTN; ADF; ACTDP; HEL32; bA462D18.2
Reactivity
Human
Synonyms
Destrin (DSTN); Human Destrin (DSTN) ELISA Kit; DSTN; ACTDP; ADF; bA462D18.2; bA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP)); destrin; DSTN elisa kit
Reactivity
Human
Specificity
Human Destrin (DSTN) ELISA Kit has high sensitivity and excellent specificity for detection of Human DSTN. No significant cross-reactivity or interference between Human DSTN and analogues was observed.
Sequence Length
148
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Destrin (DSTN) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Destrin (DSTN) Standard Curve.)
Related Product Information for DSTN elisa kit
Description: This Human Destrin (DSTN) ELISA Kit employs a two-site sandwich ELISA to quantitate DSTN in samples. An antibody specific for DSTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DSTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSTN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSTN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Destrin belongs to the actin-binding proteins ADF/cofilin family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene. In a variety of eukaryotes, destrin regulates actin in the cytoskeleton. Destrin binds actin and is thought to connect it as gelsolin segment-1 does. Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation. Destrin can also sever actin filaments. At a pH of 7.3 or higher it can sever the filaments but a lower pH the ability for it to do so is limited.
Background: Destrin belongs to the actin-binding proteins ADF/cofilin family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene. In a variety of eukaryotes, destrin regulates actin in the cytoskeleton. Destrin binds actin and is thought to connect it as gelsolin segment-1 does. Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation. Destrin can also sever actin filaments. At a pH of 7.3 or higher it can sever the filaments but a lower pH the ability for it to do so is limited.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
destrin isoform b
NCBI Official Synonym Full Names
destrin, actin depolymerizing factor
NCBI Official Symbol
DSTN
NCBI Official Synonym Symbols
ADF; ACTDP; HEL32; bA462D18.2
NCBI Protein Information
destrin
UniProt Protein Name
Destrin
Protein Family
UniProt Gene Name
DSTN
UniProt Synonym Gene Names
ACTDP; DSN; ADF
UniProt Entry Name
DEST_HUMAN
NCBI Description
The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Research Articles on DSTN
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Product Notes
The Human DSTN dstn (Catalog #AAA9715925) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715925 ELISA Kit recognizes Human DSTN. It is sometimes possible for the material contained within the vial of "Destrin (DSTN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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