Human Dentin Sialophosphoprotein (DSPP) ELISA Kit | DSPP elisa kit

Human Dentin Sialophosphoprotein (DSPP) ELISA Kit

Cat. Num. AAA2704450

• Gene Names: DSPP; DPP; DSP; DGI1; DMP3; DFNA39
• Reactivity: Human
• Synonyms: Dentin Sialophosphoprotein (DSPP); Human Dentin Sialophosphoprotein (DSPP) ELISA Kit; DSP; DFNA39; DGI1; DPP; DTDP2; DMP3; Dentin phosphoprotein; Dentin phosphophoryn; Dentin sialoprotein; DSPP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dentin Sialophosphoprotein (DSPP).; No significant cross-reactivity or interference between Dentin Sialophosphoprotein (DSPP) and analogues was observed.
• Sequence Length: 1,301

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.58ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dentin Sialophosphoprotein (DSPP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dentin Sialophosphoprotein (DSPP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DSPP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dentin Sialophosphoprotein (DSPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dentin Sialophosphoprotein (DSPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dentin Sialophosphoprotein (DSPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dentin Sialophosphoprotein (DSPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7228476
• NCBI GeneID: 1834
• UniProt Accession #: Q9NZW4; O95815; A8MUI0
• Molecular Weight: 131,151 Da
• NCBI Official Full Name: dentin sialophosphoprotein
• NCBI Official Synonym Full Names: dentin sialophosphoprotein
• NCBI Official Symbol: DSPP
• NCBI Official Synonym Symbols: DPP; DSP; DGI1; DMP3; DFNA39
• NCBI Protein Information: dentin sialophosphoprotein
• UniProt Protein Name: Dentin sialophosphoprotein
• Protein Family: Dentin sialophosphoprotein
• UniProt Gene Name: DSPP
• UniProt Synonym Gene Names: DPP; DSP

NCBI Description

This gene encodes a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of proteins. The encoded preproprotein is secreted by odontoblasts and proteolytically processed to generate two principal proteins of the dentin extracellular matrix of the tooth, dentin sialoprotein and dentin phosphoprotein. These two protein products may play distinct but related roles in dentin mineralization. Mutations in this gene are associated with dentinogenesis imperfecta and dentin dysplasia. This gene is present in a gene cluster on chromosome 4. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.