Typical Testing Data/Standard Curve (for reference only)
Human Cytosolic Fe S cluster assembly factor NUBP1 (NUBP1) ELISA Kit | NUBP1 elisa kit
Human Cytosolic Fe S cluster assembly factor NUBP1 (NUBP1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: NTX ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NTX antibody and an NTX-HRP conjugate. The assay sample and buffer are incubated together with NTX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NTX concentration since NTX from samples and NTX-HRP conjugate compete for the anti-NTX antibody binding site. Since the number of sites is limited, as more sites are occupied by NTX from the sample, fewer sites are left to bind NTX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTX concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
NUBP1 is a member of the NUBP/MRP subfamily of ATP-binding proteins (Nakashima et al., 1999 [PubMed 10486206]).[supplied by OMIM, Mar 2008]
Uniprot Description
NUBP1: Implicated in the regulation of centrosome duplication. Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. May bind and transfer 2 labile 4Fe-4S clusters to target apoproteins. Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: 16p13.13
Cellular Component: cytoplasm; plasma membrane; cytosol
Molecular Function: protein binding; metal ion binding; 4 iron, 4 sulfur cluster binding; iron-sulfur cluster binding; nucleotide binding; ATP binding
Biological Process: centrosome localization; cellular iron ion homeostasis; iron-sulfur cluster assembly; cell growth
Research Articles on NUBP1
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Product Notes
The Human NUBP1 nubp1 (Catalog #AAA7214970) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214970 ELISA Kit recognizes Human NUBP1. It is sometimes possible for the material contained within the vial of "Cytosolic Fe S cluster assembly factor NUBP1 (NUBP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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