Human Cyclin D3 (CCND3) ELISA Kit | CCND3 elisa kit

Human Cyclin D3 (CCND3) ELISA Kit

Cat. Num. AAA268546

• Reactivity: Human
• Synonyms: Cyclin D3 (CCND3); Human Cyclin D3 (CCND3) ELISA Kit; CCND3 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 292

• Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant CCND3 concentration.
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for CCND3 elisa kit

Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CCND3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 181247
• NCBI GeneID: 896
• Molecular Weight: 9,736 Da
• NCBI Official Full Name: cyclin D3
• NCBI Official Synonym Full Names: cyclin D3
• NCBI Official Symbol: CCND3
• NCBI Protein Information: G1/S-specific cyclin-D3; D3-type cyclin; G1/S-specific cyclin D3
• UniProt Protein Name: G1/S-specific cyclin-D3
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND3
• UniProt Entry Name: CCND3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

CCND3: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/p27Kip1, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K. Component of the ternary complex cyclin D/CDK4/p27Kip1 required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either p21Cip1 or CDKN2A. Belongs to the cyclin family. Cyclin D subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Cell cycle regulation; Activator

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: membrane; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus

Molecular Function: protein binding; cyclin-dependent protein kinase activity; protein kinase binding

Biological Process: T cell proliferation; positive regulation of cyclin-dependent protein kinase activity; cell division; positive regulation of protein amino acid phosphorylation; cell cycle; signal transduction