Rat Striatin (STRN) ELISA Kit | STRN elisa kit
Rat Striatin (STRN) ELISA Kit
Reactivity
Rat
Synonyms
Striatin (STRN); Rat Striatin (STRN) ELISA Kit; STRN; MGC125642; SG2NA; striatin; calmodulin-binding protein; STRN elisa kit
Reactivity
Rat
Specificity
Rat Striatin (STRN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat STRN. No significant cross-reactivity or interference between Rat STRN and analogues was observed.
Sequence Length
780
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Striatin (STRN) Standard Curve.)
Related Product Information for STRN elisa kit
Description: This Rat Striatin (STRN) ELISA Kit employs a two-site sandwich ELISA to quantitate STRN in samples. An antibody specific for STRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STRN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STRN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STRN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Striatin, a quantitatively minor protein belonging to the WD-repeat family of proteins, is a Ca2+/calmodulin-binding protein mostly expressed in the striatum and in the motor and olfactory systems. Generally associated with membranes, striatin is mostly found in dendritic spines where it is likely to play a role in Ca2+-signaling events. The predicted corresponding peptide is potentially able to form a basic amphiphilic helix, as is often the case for many known calmodulin-binding sites. Calmodulin binding to striatin is Ca2+-dependent, with half-maximal binding occurring around 0.5 mum free Ca2+. In the presence of Ca2+, the equilibrium dissociation constant of calmodulin/striatin fusion protein complex is 40 +/- 5 nm.
Background: Striatin, a quantitatively minor protein belonging to the WD-repeat family of proteins, is a Ca2+/calmodulin-binding protein mostly expressed in the striatum and in the motor and olfactory systems. Generally associated with membranes, striatin is mostly found in dendritic spines where it is likely to play a role in Ca2+-signaling events. The predicted corresponding peptide is potentially able to form a basic amphiphilic helix, as is often the case for many known calmodulin-binding sites. Calmodulin binding to striatin is Ca2+-dependent, with half-maximal binding occurring around 0.5 mum free Ca2+. In the presence of Ca2+, the equilibrium dissociation constant of calmodulin/striatin fusion protein complex is 40 +/- 5 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
striatin
NCBI Official Synonym Full Names
striatin
NCBI Official Symbol
Strn
NCBI Protein Information
striatin
UniProt Protein Name
Striatin
Protein Family
UniProt Gene Name
Strn
UniProt Entry Name
STRN_RAT
NCBI Description
calmodulin-binding protein [RGD, Feb 2006]
