Human Cyclin D3 (CCND3) ELISA Kit | CCND3 elisa kit

Human Cyclin D3 (CCND3) ELISA Kit

Cat. Num. AAA2088133

• Reactivity: Human
• Synonyms: Cyclin D3 (CCND3); Human Cyclin D3 (CCND3) ELISA Kit; CCND3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclin D3 (CCND3).; No significant cross-reactivity or interference between Cyclin D3 (CCND3) and analogues was observed.
• Sequence Length: 211

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin D3 (CCND3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin D3 (CCND3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCND3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin D3 (CCND3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin D3 (CCND3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin D3 (CCND3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin D3 (CCND3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CCND3 elisa kit

Tumor immunity; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 209862835
• NCBI GeneID: 896
• NCBI Accession #: NP_001129489.1
• NCBI GenBank Nucleotide #: NM_001136017.3
• UniProt Accession #: P30281; Q5T8J0; Q6FG62; Q96F49; B2RD63; B3KQ22; E9PAS4; E9PB36
• Molecular Weight: 9,736 Da
• NCBI Official Full Name: G1/S-specific cyclin-D3 isoform 1
• NCBI Official Synonym Full Names: cyclin D3
• NCBI Official Symbol: CCND3
• NCBI Protein Information: G1/S-specific cyclin-D3
• UniProt Protein Name: G1/S-specific cyclin-D3
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND3

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

CCND3: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/p27Kip1, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K. Component of the ternary complex cyclin D/CDK4/p27Kip1 required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either p21Cip1 or CDKN2A. Belongs to the cyclin family. Cyclin D subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Activator; Cell cycle regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytoplasm; focal adhesion; nucleoplasm; plasma membrane

Molecular Function: protein binding; protein kinase binding

Biological Process: positive regulation of cyclin-dependent protein kinase activity; positive regulation of protein amino acid phosphorylation