Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cullin-9 (CUL9) Standard Curve.)
Human Cullin-9 (CUL9) ELISA Kit | CUL9 elisa kit
Human Cullin-9 (CUL9) ELISA Kit
Gene Names
CUL9; PARC; H7AP1
Reactivity
Human
Synonyms
Cullin-9 (CUL9); Human Cullin-9 (CUL9) ELISA Kit; CUL9; DKFZp686G1042; DKFZp686P2024; H7AP1; PARC; RP3-330M21.2; UbcH7-associated protein 1; p53-associated parkin-like cytoplasmic protein; parkin-like cytoplasmic p53 binding protein; CUL9 elisa kit
Reactivity
Human
Specificity
Human Cullin-9 (CUL9) ELISA Kit has high sensitivity and excellent specificity for detection of Human CUL9. No significant cross-reactivity or interference between Human CUL9 and analogues was observed.
Sequence Length
2517
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cullin-9 (CUL9) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cullin-9 (CUL9) Standard Curve.)
Related Product Information for CUL9 elisa kit
Description: This Human Cullin-9 (CUL9) ELISA Kit employs a two-site sandwich ELISA to quantitate CUL9 in samples. An antibody specific for CUL9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CUL9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CUL9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CUL9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PARC directly interacted and formed an approximately 1-MD complex with p53 in the cytoplasm of unstressed cells. In the absence of stress, inactivation of PARC induced nuclear localization of endogenous p53 and activated p53-dependent apoptosis. Overexpression of PARC promoted cytoplasmic sequestration of ectopic p53.abnormal cytoplasmic localization of p53 was observed in a number of neuroblastoma cell lines; RNA interference-mediated reduction of endogenous PARC significantly sensitized these neuroblastoma cells in the DNA damage response. These results revealed that PARC is a critical regulator in controlling p53 subcellular localization and subsequent function.PARC also has a unique motif that is highly homologous to the C terminus of cullin proteins that the authors referred to as the C-terminal cullin homology domain.
Background: PARC directly interacted and formed an approximately 1-MD complex with p53 in the cytoplasm of unstressed cells. In the absence of stress, inactivation of PARC induced nuclear localization of endogenous p53 and activated p53-dependent apoptosis. Overexpression of PARC promoted cytoplasmic sequestration of ectopic p53.abnormal cytoplasmic localization of p53 was observed in a number of neuroblastoma cell lines; RNA interference-mediated reduction of endogenous PARC significantly sensitized these neuroblastoma cells in the DNA damage response. These results revealed that PARC is a critical regulator in controlling p53 subcellular localization and subsequent function.PARC also has a unique motif that is highly homologous to the C terminus of cullin proteins that the authors referred to as the C-terminal cullin homology domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cullin-9
NCBI Official Synonym Full Names
cullin 9
NCBI Official Symbol
CUL9
NCBI Official Synonym Symbols
PARC; H7AP1
NCBI Protein Information
cullin-9
UniProt Protein Name
Cullin-9
Protein Family
UniProt Gene Name
CUL9
UniProt Synonym Gene Names
H7AP1; KIAA0708; PARC; CUL-9
UniProt Entry Name
CUL9_HUMAN
Uniprot Description
PARC: Cytoplasmic anchor protein in p53-associated protein complex. Regulates the subcellular localization of p53 and subsequent function. Seems to be part of an atypical cullin-RING- based E3 ubiquitin-protein ligase complex. In vitro, complexes of CUL9/PARC with either CUL7 or TP53 contain E3 ubiquitin-protein ligase activity. Belongs to the cullin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: cullin-RING ubiquitin ligase complex; cytoplasm
Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; ATP binding
Biological Process: ubiquitin-dependent protein catabolic process; regulation of mitosis; protein ubiquitination; microtubule cytoskeleton organization and biogenesis
Research Articles on CUL9
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Product Notes
The Human CUL9 cul9 (Catalog #AAA9716076) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716076 ELISA Kit recognizes Human CUL9. It is sometimes possible for the material contained within the vial of "Cullin-9 (CUL9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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