Human Cubilin (CUBN) ELISA Kit | CUBN elisa kit

Human Cubilin (CUBN) ELISA Kit

Cat. Num. AAA2021105

• Gene Names: Cubn; IFCR; MGA1; GP280
• Reactivity: Human
• Synonyms: Cubilin (CUBN); Human Cubilin (CUBN) ELISA Kit; IFCR; MGA1; gp280; Intrinsic Factor Cobalamin Receptor; 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-vitamin B12 receptor; CUBN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cubilin (CUBN).; No significant cross-reactivity or interference between Cubilin (CUBN) and analogues was observed.
• Sequence Length: 3623

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cubilin (CUBN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cubilin (CUBN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CUBN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cubilin (CUBN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cubilin (CUBN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cubilin (CUBN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cubilin (CUBN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CUBN elisa kit

Signal transduction; Metabolic pathway; Nutrition metabolism; Dermatology

NCBI and Uniprot Product Information

• NCBI GI #: 16758040
• NCBI GeneID: 80848
• NCBI Accession #: NP_445784.1
• NCBI GenBank Nucleotide #: NM_053332.2
• UniProt Accession #: O70244
• Molecular Weight: 398,987 Da
• NCBI Official Full Name: cubilin
• NCBI Official Synonym Full Names: cubilin (intrinsic factor-cobalamin receptor)
• NCBI Official Symbol: Cubn
• NCBI Official Synonym Symbols: IFCR; MGA1; GP280
• NCBI Protein Information: cubilin
• UniProt Protein Name: Cubilin
• Protein Family: Cubilin
• UniProt Gene Name: Cubn
• UniProt Synonym Gene Names: Ifcr; gp280
• UniProt Entry Name: CUBN_RAT

NCBI Description

receptor for intrinsic factor (IF)-cobalamin; involved in vitamin B12 uptake (cyanocobalamin) [RGD, Feb 2006]

Uniprot Description

CUBN: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected.

Cellular Component: Golgi apparatus; lysosomal lumen; protein complex; lysosomal membrane; endoplasmic reticulum; brush border membrane; coated pit; membrane; apical part of cell; endocytic vesicle; cytoplasm; apical plasma membrane; endosome membrane; brush border; endosome

Molecular Function: identical protein binding; protein homodimerization activity; hemoglobin binding; cobalamin transporter activity; calcium ion binding; receptor activity; cobalamin binding

Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; vitamin metabolic process; in utero embryonic development; lipoprotein transport; cobalamin transport; hemoglobin import; response to nutrient