Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Anti-CSFV IgM Antibody (CSFV-Ab-IgM) Standard Curve.)
Human CSFV IgM (CSFV-Ab-IgM) ELISA Kit | CSFV-Ab-IgM elisa kit
Human Anti-CSFV IgM Antibody (CSFV-Ab-IgM) ELISA Kit
Reactivity
Human
Synonyms
CSFV IgM (CSFV-Ab-IgM); Human Anti-CSFV IgM Antibody (CSFV-Ab-IgM) ELISA Kit; CSFV-Ab-IgM elisa kit
Reactivity
Human
Specificity
Human Anti-CSFV IgM Antibody (CSFV-Ab-IgM) ELISA Kit has high sensitivity and excellent specificity for detection of Human CSFV-Ab-IgM. No significant cross-reactivity or interference between Human CSFV-Ab-IgM and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Anti-CSFV IgM Antibody (CSFV-Ab-IgM) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Anti-CSFV IgM Antibody (CSFV-Ab-IgM) Standard Curve.)
Related Product Information for CSFV-Ab-IgM elisa kit
Description: This Human Anti-CSFV IgM Antibody (CSFV-Ab-IgM) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CSFV-Ab-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated CSFV-Ab-IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled CSFV-Ab-IgM and unlabeled CSFV-Ab-IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of CSFV-Ab-IgM in the sample. The color development is stopped and the intensity of the color is measured.
Background: Classical Swine Fever Virus (CSFV), Bovine Viral Diarrhoea Virus (BVDV), and Border Disease Virus (BDV) are the 3 members of the genus Pestivirus within the family Flaviviridae. CSFV causes serious losses in the pig industry since it is highly contagious and pathogenic, and can cause widespread deaths. Pigs infected with CSFV may shed a high amount of virus before showing clinical signs of the disease. If animals survive an acute or sub-acute infection they develop antibodies or become chronically infected, a situation where pigs excrete the virus intermittently or continuously until death. In pregnant sows, CSFV is able to cross the placenta and infect fetuses. It causes abortions, fetal mummification and stillborns; if the infection occurs mid gestation (~50-70 days of pregnancy), weak or persistently viremic piglets can be born. These persistently infected piglets are able to spread the virus, due to the absence of an immune response. Occasionally pigs may be infected with BVDV or BDV. These infections in pigs are usually mild and self-limiting, but it is important to identify them from the CSFV infection.
Background: Classical Swine Fever Virus (CSFV), Bovine Viral Diarrhoea Virus (BVDV), and Border Disease Virus (BDV) are the 3 members of the genus Pestivirus within the family Flaviviridae. CSFV causes serious losses in the pig industry since it is highly contagious and pathogenic, and can cause widespread deaths. Pigs infected with CSFV may shed a high amount of virus before showing clinical signs of the disease. If animals survive an acute or sub-acute infection they develop antibodies or become chronically infected, a situation where pigs excrete the virus intermittently or continuously until death. In pregnant sows, CSFV is able to cross the placenta and infect fetuses. It causes abortions, fetal mummification and stillborns; if the infection occurs mid gestation (~50-70 days of pregnancy), weak or persistently viremic piglets can be born. These persistently infected piglets are able to spread the virus, due to the absence of an immune response. Occasionally pigs may be infected with BVDV or BDV. These infections in pigs are usually mild and self-limiting, but it is important to identify them from the CSFV infection.
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Product Notes
The Human CSFV-Ab-IgM (Catalog #AAA9716157) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716157 ELISA Kit recognizes Human CSFV-Ab-IgM. It is sometimes possible for the material contained within the vial of "CSFV IgM (CSFV-Ab-IgM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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