Human CRP (C-Reactive Protein) ELISA Kit | CRP elisa kit

QuicKey Pro Human CRP (C-Reactive Protein) ELISA Kit

Cat. Num. AAA2568086

• Gene Names: CRP; PTX1
• Reactivity: Human
• Synonyms: CRP (C-Reactive Protein); QuicKey Pro Human CRP (C-Reactive Protein) ELISA Kit; C-Reactive Protein; CRP; PTX1; CRP elisa kit

• Reactivity: Human
• Specificity: This kit recognizes CRP in samples. No significant cross-reactivity or interference between CRP and analogues was observed.; This ELISA kit applies to the in vitro quantitative determination of CRP concentrations in serum and plasma.

• Assay Type: Sandwich ELISA
• Detection Method: Colormetric
• Tested Sample Types: Serum and plasma
• Detection Range: 125-8000pg/mL
• Sensitivity: 52.1pg/mL
• Precision: Both intra-CV and inter-CV are < 10%.
• Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CRP are added to the micro ELISA plate wells. Human CRP in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to CRP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450+/-2nm. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
• Preparation and Storage: An unopened kit can be stored at 2-8? for 6 months. After test, the unused wells and reagents should be stored according to the table/manual.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 55770842
• NCBI GeneID: 1401
• NCBI Accession #: NP_000558.2
• NCBI GenBank Nucleotide #: NM_000567.2
• UniProt Accession #: P02741; Q08AK3; Q8WW75; A8K078; D3DVD9; D3DVE0
• Molecular Weight: 25,039 Da
• NCBI Official Full Name: C-reactive protein
• NCBI Official Synonym Full Names: C-reactive protein, pentraxin-related
• NCBI Official Symbol: CRP
• NCBI Official Synonym Symbols: PTX1
• NCBI Protein Information: C-reactive protein; pentraxin 1
• UniProt Protein Name: C-reactive protein
• Protein Family: Cryparin
• UniProt Gene Name: CRP
• UniProt Synonym Gene Names: PTX1
• UniProt Entry Name: CRP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

Uniprot Description

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: extracellular space; growth cone; extracellular region; filopodium

Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; cholesterol binding; low-density lipoprotein binding; virion binding; calcium ion binding

Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; negative regulation of vasodilation; wound healing; positive regulation of superoxide release; response to lead ion; response to hypoxia; acute-phase response; opsonization; inflammatory response; complement activation, classical pathway; aging