Human CRLF2/TSLP R ELISA Kit | CRLF2 elisa kit

Human CRLF2/TSLP R PicoKine ELISA Kit

Cat. Num. AAA177284

• Gene Names: CRLF2; CRL2; TSLPR; CRLF2Y
• Reactivity: Human
• Synonyms: CRLF2/TSLP R; Human CRLF2/TSLP R PicoKine ELISA Kit; Cytokine receptor-like factor 2; cytokine receptor-like factor 2; CRL 2; CRL2; CRLF 2; Crlf2; CRLF2_HUMAN; CRLF2Y; Cytokine receptor CRL2 precusor; Cytokine receptor like 2; Cytokine receptor like factor 2; Cytokine receptor like factor 2 precursor; Cytokine receptor-like 2; IL XR; IL-XR; ILXR; Thymic stromal derived lymphopoietin receptor; Thymic stromal lymphopoietin protein receptor; Thymic stromal lymphopoietin receptor; TSLP receptor; TSLPR; CRLF2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CRLF2
• Sequence Length: 371

• Samples: Cell culture supernates, serum, and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human CRLF2/TSLP R PicoKine ELISA Kit standard curve )

Related Product Information for CRLF2 elisa kit

Principle of the assay: human CRLF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRLF2 has been precoated onto 96-well plates. Standards(NSO, G25-K231) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRLF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CRLF2 amount of sample captured in plate.
Background: Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin(TSLP). Together with the interleukin 7 receptor(IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.

NCBI and Uniprot Product Information

• NCBI GI #: 38257768
• NCBI GeneID: 64109
• UniProt Accession #: Q9HC73; Q9H5R3
• Molecular Weight: 26,774 Da
• NCBI Official Full Name: Cytokine receptor-like factor 2
• NCBI Official Synonym Full Names: cytokine receptor-like factor 2
• NCBI Official Symbol: CRLF2
• NCBI Official Synonym Symbols: CRL2; TSLPR; CRLF2Y
• NCBI Protein Information: cytokine receptor-like factor 2; IL-XR; TSLP receptor; cytokine receptor CRL2 precusor; thymic stromal lymphopoietin protein receptor; thymic stromal-derived lymphopoietin receptor
• UniProt Protein Name: Cytokine receptor-like factor 2
• Protein Family: Cytokine receptor-like factor
• UniProt Gene Name: CRLF2
• UniProt Synonym Gene Names: CRL2; ILXR; TSLPR; TSLP receptor
• UniProt Entry Name: CRLF2_HUMAN

NCBI Description

This gene encodes a member of the type I cytokine receptor family. The encoded protein is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CRLF2: Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3; Yp11.3

Cellular Component: plasma membrane; extracellular region; integral to membrane