Human Creatine Kinase, Mitochondrial 1A (CKMT1A) ELISA Kit | CKMT1A elisa kit

Human Creatine Kinase, Mitochondrial 1A (CKMT1A) ELISA Kit

Cat. Num. AAA454456

• Gene Names: CKMT1A; CKMT1; U-MtCK; mia-CK
• Reactivity: Human
• Synonyms: Creatine Kinase; Mitochondrial 1A (CKMT1A); Human Creatine Kinase; Mitochondrial 1A (CKMT1A) ELISA Kit; CKMT CKMT1-A Mia-CK U-MtCK Creatine kinase U-type; mitochondrial Ubiquitous mitochondrial creatine kinase Acidic-type mitochondrial creatine kinase; CKMT1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CKMT1A. No significant cross-reactivity or interference between CKMT1A and analogues was observed.
• Sequence Length: 417

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.57ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CKMT1A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CKMT1A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CKMT1A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CKMT1A in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CKMT1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CKMT1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CKMT1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CKMT1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 66346730
• NCBI GeneID: 548596
• NCBI Accession #: NP_001015001.1
• NCBI GenBank Nucleotide #: NM_001015001.2
• UniProt Accession #: P12532; Q0VAM3; Q32NF6; Q53FC4; B4DIT8; B7ZA09
• Molecular Weight: 50,421 Da
• NCBI Official Full Name: creatine kinase U-type, mitochondrial isoform 1
• NCBI Official Synonym Full Names: creatine kinase, mitochondrial 1A
• NCBI Official Symbol: CKMT1A
• NCBI Official Synonym Symbols: CKMT1; U-MtCK; mia-CK
• NCBI Protein Information: creatine kinase U-type, mitochondrial
• UniProt Protein Name: Creatine kinase U-type, mitochondrial
• UniProt Gene Name: CKMT1A
• UniProt Synonym Gene Names: CKMT; Mia-CK; U-MtCK

NCBI Description

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.