Human Coxsackievirus IgG ELISA Kit | IgG elisa kit
Coxsackievirus IgG ELISA Kit
Reactivity
Human
Synonyms
Coxsackievirus IgG; Coxsackievirus IgG ELISA Kit; IgG elisa kit
Reactivity
Human
Specificity
The evaluation of the Chlamydia trachomatis IgA, IgG, and IgM tests was performed in an internal study. The sensitivity exceeded 93% and specificity exceeded 95%.
Samples
Serum Or Plasma Directed Against Chlamydia Trachomatis.
Preparation and Storage
2 - 8 degree C
Related Product Information for IgG elisa kit
Intended Uses: The ELISA Chlamydia trachomatis IgA, IgG and IgM tests are qualitative immunoassays for the detection of human IgA, IgG, and IgM antibodies in serum or plasma directed against Chlamydia trachomatis. The differential detection of the different antibody classes facilitates the confirmation of contact with the organism and supports the categorisation of the disease stage.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the serum sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate p-nitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: Members of the order Chlamydiales are gram negative, intracellular bacteria. Chlamydia trachomatis, Chlamydia pneumoniae (Chlamydophila pneumoniae) and Chlamydia psittaci (Chlamydophila psittaci) are the relevant human pathogens. Chlamydia alternates between two morphological forms during its lifecycle, the elementary bodies (EB) and the reticular bodies (RB). The elementary bodies represent the extracellular and metabolically inert stage responsible for infection. Once inside the host-cell, the bacteria transform to the metabolically active reticular bodies. When the reproduction cycle has finished, the bacteria differentiate back to elementary bodies and leave the host. Chlamydia trachomatis is one of the most common sexually transmitted procaryotic pathogens. The bacterium infects preliminary epithelial cells of the urogenital-and respiratory tract as well as cells of the conjunctiva. The World Health Organization (WHO) estimates, that 90 million people annually become infected by Chlamydia trachomatis. According to the Robert Koch Institute (RKI), the prevalence in the European population with this pathogen is approximately 4 to 6 %. Chlamydia trachomatis infections may be asymptomatic in 70 % of females and in up to 50 % of males. Untreated, infections can result in serious damage and complications. The serovars A to C cause ceratoconjunctivits. Chronic infections during childhood can result in trachoma or blindness. Serovars D to K are pathogens of the urogenital tract, responsible for urethritis, proctitis and cervicitis. Salpinigits, endomitritis and perihepatitis are frequently the consequence of untreated cervicitis. Occasionally, fallopian tube obstructions and ectopic pregnancy, which are one of the most common reasons for infertility in women, may result. Furthermore, the risk of a premature delivery for infected pregnant women is also increased. The risk of transfer to the newborn during parturition is around 60% with conjunctivitis or pneumonia as possible sequelae. In addition to urethritis and proctitis, epidiymitis and prostatitis, which may lead to infertility, are possible consequences for men. The serovars L1 to L3 spread systematically through the lymphatic tissue causing the invasive disease known as lymphogranuloma venereum. Stricture and stenosis are the consequences, which have to be treated surgically. In cases of newborn chlamydiosis, circulating plasma-and B-cells generate IgM antibodies in high concentrations. Due to the fact that IgM antibodies are produced earlier than IgG and IgA, they are of higher significance in neonatal. Mahoney and co-workers demonstrated that infected newborns displayed detectable IgM titres within 5 days after contact with the pathogen. The ELISA Chlamydia trachomatis tests are based on a specific domain of the major outer membrane protein (MOMP). The use of this antigen decreases the frequency of false-positive results and consequently increases the specificity of the antibody detection in comparison to Microimmunofluorescence tests and other ELISAs.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the serum sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate p-nitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: Members of the order Chlamydiales are gram negative, intracellular bacteria. Chlamydia trachomatis, Chlamydia pneumoniae (Chlamydophila pneumoniae) and Chlamydia psittaci (Chlamydophila psittaci) are the relevant human pathogens. Chlamydia alternates between two morphological forms during its lifecycle, the elementary bodies (EB) and the reticular bodies (RB). The elementary bodies represent the extracellular and metabolically inert stage responsible for infection. Once inside the host-cell, the bacteria transform to the metabolically active reticular bodies. When the reproduction cycle has finished, the bacteria differentiate back to elementary bodies and leave the host. Chlamydia trachomatis is one of the most common sexually transmitted procaryotic pathogens. The bacterium infects preliminary epithelial cells of the urogenital-and respiratory tract as well as cells of the conjunctiva. The World Health Organization (WHO) estimates, that 90 million people annually become infected by Chlamydia trachomatis. According to the Robert Koch Institute (RKI), the prevalence in the European population with this pathogen is approximately 4 to 6 %. Chlamydia trachomatis infections may be asymptomatic in 70 % of females and in up to 50 % of males. Untreated, infections can result in serious damage and complications. The serovars A to C cause ceratoconjunctivits. Chronic infections during childhood can result in trachoma or blindness. Serovars D to K are pathogens of the urogenital tract, responsible for urethritis, proctitis and cervicitis. Salpinigits, endomitritis and perihepatitis are frequently the consequence of untreated cervicitis. Occasionally, fallopian tube obstructions and ectopic pregnancy, which are one of the most common reasons for infertility in women, may result. Furthermore, the risk of a premature delivery for infected pregnant women is also increased. The risk of transfer to the newborn during parturition is around 60% with conjunctivitis or pneumonia as possible sequelae. In addition to urethritis and proctitis, epidiymitis and prostatitis, which may lead to infertility, are possible consequences for men. The serovars L1 to L3 spread systematically through the lymphatic tissue causing the invasive disease known as lymphogranuloma venereum. Stricture and stenosis are the consequences, which have to be treated surgically. In cases of newborn chlamydiosis, circulating plasma-and B-cells generate IgM antibodies in high concentrations. Due to the fact that IgM antibodies are produced earlier than IgG and IgA, they are of higher significance in neonatal. Mahoney and co-workers demonstrated that infected newborns displayed detectable IgM titres within 5 days after contact with the pathogen. The ELISA Chlamydia trachomatis tests are based on a specific domain of the major outer membrane protein (MOMP). The use of this antigen decreases the frequency of false-positive results and consequently increases the specificity of the antibody detection in comparison to Microimmunofluorescence tests and other ELISAs.
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Product Notes
The Human IgG (Catalog #AAA495309) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495309 ELISA Kit recognizes Human IgG. It is sometimes possible for the material contained within the vial of "Coxsackievirus IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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