Human Corneodesmosin (CDSN) ELISA Kit | CDSN elisa kit

Human Corneodesmosin (CDSN) ELISA Kit

Cat. Num. AAA2024938

• Gene Names: CDSN; S; PSS; HTSS; PSS1; HTSS1; HYPT2
• Reactivity: Human
• Synonyms: Corneodesmosin (CDSN); Human Corneodesmosin (CDSN) ELISA Kit; HTSS; S protein; CDSN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Corneodesmosin (CDSN).; No significant cross-reactivity or interference between Corneodesmosin (CDSN) and analogues was observed.
• Sequence Length: 529

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corneodesmosin (CDSN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corneodesmosin (CDSN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDSN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corneodesmosin (CDSN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corneodesmosin (CDSN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corneodesmosin (CDSN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corneodesmosin (CDSN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CDSN elisa kit

Metabolic pathway; Dermatology

NCBI and Uniprot Product Information

• NCBI GI #: 67782356
• NCBI GeneID: 1041
• NCBI Accession #: NP_001255.3
• NCBI GenBank Nucleotide #: NM_001264.4
• Molecular Weight: 51,522 Da
• NCBI Official Full Name: corneodesmosin
• NCBI Official Synonym Full Names: corneodesmosin
• NCBI Official Symbol: CDSN
• NCBI Official Synonym Symbols: S; PSS; HTSS; PSS1; HTSS1; HYPT2
• NCBI Protein Information: corneodesmosin
• UniProt Protein Name: Corneodesmosin
• Protein Family: Corneodesmosin
• UniProt Gene Name: CDSN
• UniProt Entry Name: CDSN_HUMAN

NCBI Description

This gene encodes a protein found in corneodesmosomes, which localize to human epidermis and other cornified squamous epithelia. The encoded protein undergoes a series of cleavages during corneocyte maturation. This gene is highly polymorphic in human populations, and variation has been associated with skin diseases such as psoriasis, hypotrichosis and peeling skin syndrome. The gene is located in the major histocompatibility complex (MHC) class I region on chromosome 6. [provided by RefSeq, Dec 2014]

Uniprot Description

CDSN: Important for the epidermal barrier integrity. Defects in CDSN are the cause of hypotrichosis type 2 (HYPT2). A condition characterized by the presence of less than the normal amount of hair. Affected individuals have normal hair in early childhood but experience progressive hair loss limited to the scalp beginning in the middle of the first decade and almost complete baldness by the third decade. Body hair, beard, eyebrows, axillary hair, teeth, and nails develop normally. Defects in CDSN are a cause of peeling skin syndrome (PSS); also known as peeling skin syndrome or deciduous skin or keratolysis exfoliativa congenita. A genodermatosis characterized by generalized, continuous shedding of the outer layers of the epidermis. Two main PSS subtypes have been suggested. Patients with non-inflammatory PSS (type A) manifest white scaling, with painless and easy removal of the skin, irritation when in contact with water, dust and sand, and no history of erythema, pruritis or atopy. Inflammatory PSS (type B) is associated with generalized erythema, pruritus and atopy. It is an ichthyosiform erythroderma characterized by lifelong patchy peeling of the entire skin with onset at birth or shortly after. Several patients have been reported with high IgE levels. CDNS mutations are responsible for generalized, inflammatory peeling skin syndrome type B (PubMed:20691404).

Protein type: Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: desmosome; cornified envelope; intercellular junction

Molecular Function: protein homodimerization activity

Biological Process: keratinocyte differentiation; epidermis development; cell-cell adhesion; skin morphogenesis; cell adhesion

Disease: Peeling Skin Syndrome 1; Hypotrichosis 2