Human Contactin 4 (CNTN4) ELISA Kit | CNTN4 elisa kit

Human Contactin 4 (CNTN4) ELISA Kit

Cat. Num. AAA2020449

• Gene Names: CNTN4; AXCAM; BIG-2
• Reactivity: Human
• Synonyms: Contactin 4 (CNTN4); Human Contactin 4 (CNTN4) ELISA Kit; AXCAM; BIG-2; CNTN4A; Brain-derived immunoglobulin superfamily protein 2; CNTN4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Contactin 4 (CNTN4).; No significant cross-reactivity or interference between Contactin 4 (CNTN4) and analogues was observed.
• Sequence Length: 1026

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Contactin 4 (CNTN4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Contactin 4 (CNTN4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNTN4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Contactin 4 (CNTN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Contactin 4 (CNTN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Contactin 4 (CNTN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Contactin 4 (CNTN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CNTN4 elisa kit

Tumor immunity; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 332688245
• NCBI GeneID: 152330
• NCBI Accession #: NP_001193884.1
• NCBI GenBank Nucleotide #: NM_001206955.1
• UniProt Accession #: Q8IWV2; Q8IX14; Q8TC35; B2RAX3
• Molecular Weight: 76,655 Da
• NCBI Official Full Name: contactin-4 isoform a
• NCBI Official Synonym Full Names: contactin 4
• NCBI Official Symbol: CNTN4
• NCBI Official Synonym Symbols: AXCAM; BIG-2
• NCBI Protein Information: contactin-4
• UniProt Protein Name: Contactin-4
• Protein Family: Contactin
• UniProt Gene Name: CNTN4
• UniProt Synonym Gene Names: BIG-2
• UniProt Entry Name: CNTN4_HUMAN

NCBI Description

This gene encodes a member of the contactin family of immunoglobulins. Contactins are axon-associated cell adhesion molecules that function in neuronal network formation and plasticity. The encoded protein is a glycosylphosphatidylinositol-anchored neuronal membrane protein that may play a role in the formation of axon connections in the developing nervous system. Deletion or mutation of this gene may play a role in 3p deletion syndrome and autism spectrum disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011]

Uniprot Description

CNTN4: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis. A chromosomal aberration involving CNTN4 has been found in a boy with characteristic physical features of 3p deletion syndrome (3PDS). Translocation t(3;10)(p26;q26). 3PDS is a rare contiguous gene disorder involving the loss of the telomeric portion of the short arm of chromosome 3 and characterized by developmental delay, growth retardation, and dysmorphic features. Belongs to the immunoglobulin superfamily. Contactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3p26.3

Cellular Component: axon; extracellular region; plasma membrane

Biological Process: nervous system development; axon guidance; negative regulation of neuron differentiation; axonogenesis; axonal fasciculation; regulation of synaptic plasticity; neuron adhesion; brain development; neurite development