Rat Contactin 4 ELISA Kit | CNTN4 elisa kit

Rat Contactin 4 ELISA Kit

Cat. Num. AAA760673

• Reactivity: Rat
• Synonyms: Contactin 4; Rat Contactin 4 ELISA Kit; CNTN4/Brain-derived immunoglobulin superfamily protein 2 (BIG-2)/BIG-2/CNTN4/AXCAM/axonal-associated cell adhesion molecule/BIG-2Brain-derived immunoglobulin superfamily protein 2/contactin 4/contactin-4/neural cell adhesion protein BIG-2; CNTN4 elisa kit

• Reactivity: Rat
• Sequence Length: 1026

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CNTN4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat CNTN4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat CNTN4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat CNTN4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat CNTN4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 26891535
• Molecular Weight: 76,655 Da
• NCBI Official Full Name: contactin 4
• UniProt Protein Name: Contactin-4
• Protein Family: Contactin
• UniProt Gene Name: CNTN4
• UniProt Synonym Gene Names: BIG-2
• UniProt Entry Name: CNTN4_HUMAN

Uniprot Description

CNTN4: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis. A chromosomal aberration involving CNTN4 has been found in a boy with characteristic physical features of 3p deletion syndrome (3PDS). Translocation t(3;10)(p26;q26). 3PDS is a rare contiguous gene disorder involving the loss of the telomeric portion of the short arm of chromosome 3 and characterized by developmental delay, growth retardation, and dysmorphic features. Belongs to the immunoglobulin superfamily. Contactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3p26.3

Cellular Component: axon; extracellular region; plasma membrane

Biological Process: axon guidance; axonal fasciculation; axonogenesis; brain development; negative regulation of neuron differentiation; nervous system development; neurite development; neuron adhesion; regulation of synaptic plasticity