Human Contactin 1 (CNTN1) ELISA Kit | CNTN1 elisa kit

Human Contactin 1 (CNTN1) ELISA Kit

Cat. Num. AAA2703006

• Gene Names: Cntn1; usl; CNTN; F3cam; AW495098
• Reactivity: Human
• Synonyms: Contactin 1 (CNTN1); Human Contactin 1 (CNTN1) ELISA Kit; F3; Glycoprotein gP135; Neural cell surface protein F3; CNTN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Contactin 1 (CNTN1).; No significant cross-reactivity or interference between Contactin 1 (CNTN1) and analogues was observed.
• Sequence Length: 1020

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Contactin 1 (CNTN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Contactin 1 (CNTN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNTN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Contactin 1 (CNTN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Contactin 1 (CNTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Contactin 1 (CNTN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Contactin 1 (CNTN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148672348
• NCBI GeneID: 12805
• Molecular Weight: 113,388 Da
• NCBI Official Full Name: contactin 1
• NCBI Official Synonym Full Names: contactin 1
• NCBI Official Symbol: Cntn1
• NCBI Official Synonym Symbols: usl; CNTN; F3cam; AW495098
• NCBI Protein Information: contactin-1
• UniProt Protein Name: Contactin-1
• Protein Family: Contactin
• UniProt Gene Name: Cntn1

Uniprot Description

CNTN1: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM). CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia. Belongs to the immunoglobulin superfamily. Contactin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 15 E3|15 46.39 cM

Cellular Component: membrane; membrane raft; myelin sheath; plasma membrane

Molecular Function: carbohydrate binding; protein binding

Biological Process: cell adhesion; cerebellum development; nervous system development; neuron projection development; Notch signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation