Typical Testing Data/Standard Curve (for reference only)
Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit | HNF1b elisa kit
Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b)(Sandwich ELISA) ELISA Kit
Reactivity
Rat
Applications
ELISA
Synonyms
Hepatocyte Nuclear Factor 1 Beta (HNF1b); Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b)(Sandwich ELISA) ELISA Kit; Mini Samples ELISA Kit for Hepatocyte Nuclear Factor 1 Beta (HNF1b); HNF1b/Hepatocyte Nuclear Factor 1 Beta; FJHN; TCF2; HNF1beta; HNF2; LFB3; LF-B3; MODY5; VHNF1; Transcription Factor 2; Hepatic; Variant Hepatic Nuclear Factor; Homeoprotein LFB3; HNF1b elisa kit
Reactivity
Rat
Specificity
Reactive with Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b)
This assay has high sensitivity and excellent specificity for detection of Mini Samples Hepatocyte Nuclear Factor 1 Beta (HNF1b). No significant cross-reactivity or interference between Mini Samples Hepatocyte Nuclear Factor 1 Beta (HNF1b) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Mini Samples Hepatocyte Nuclear Factor 1 Beta (HNF1b). No significant cross-reactivity or interference between Mini Samples Hepatocyte Nuclear Factor 1 Beta (HNF1b) and analogues was observed.
Applicable Applications for HNF1b elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Type
Sandwich ELISA-Mini Samples
Sample Types
tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.059ng/mL
Detection Method
Double-antibody Sandwich
Assay Length
3 hours
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 7 (GAL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 7 (GAL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 7 (GAL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Galectin 7 (GAL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 25ul standard or sample to each well. Incubate 1 hour at 37 degree C
3. Aspirate and add 25ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 25ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 25ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 20ul Stop Solution. Read at 450nm immediately.
2. Add 25ul standard or sample to each well. Incubate 1 hour at 37 degree C
3. Aspirate and add 25ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 25ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 25ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 20ul Stop Solution. Read at 450nm immediately.
Preparation and Storage
Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for HNF1b elisa kit
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Product Notes
The Rat HNF1b (Catalog #AAA2125306) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2125306 ELISA Kit recognizes Rat HNF1b. AAA Biotech's Hepatocyte Nuclear Factor 1 Beta (HNF1b) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the HNF1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepatocyte Nuclear Factor 1 Beta (HNF1b), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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