Typical Testing Data/Standard Curve (for reference only)
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Human Complement C1q subcomponent subunit A (C1QA) ELISA Kit | C1QA elisa kit
Human Complement C1q subcomponent subunit A (C1QA) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Principle of the Assay: C1QA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-C1QA antibody and an C1QA-HRP conjugate. The assay sample and buffer are incubated together with C1QA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C1QA concentration since C1QA from samples and C1QA-HRP conjugate compete for the anti-C1QA antibody binding site. Since the number of sites is limited, as more sites are occupied by C1QA from the sample, fewer sites are left to bind C1QA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1QA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q. [provided by RefSeq, Jul 2008]
Uniprot Description
C1QA iso2: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2 )-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1p36.12
Cellular Component: collagen; extracellular region; complement component C1 complex
Molecular Function: protein binding
Biological Process: cell-cell signaling; innate immune response; complement activation, classical pathway; complement activation
Disease: C1q Deficiency
Research Articles on C1QA
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Product Notes
The Human C1QA c1qa (Catalog #AAA7227334) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7227334 ELISA Kit recognizes Human C1QA. It is sometimes possible for the material contained within the vial of "Complement C1q subcomponent subunit A (C1QA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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