Human Collagen alpha-1 (EMID2) ELISA Kit | EMID2 elisa kit

Human Collagen alpha-1 (EMID2) ELISA Kit

Cat. Num. AAA2801777

• Gene Names: COL26A1; EMI6; EMU2; SH2B; EMID2
• Reactivity: Human
• Synonyms: Collagen alpha-1 (EMID2); Human Collagen alpha-1 (EMID2) ELISA Kit; COL26A1; EMI6; EMU2; MGC129848; SH2B; hEmu2; Emu2; collagen; type XXVI; alpha 1; emilin and multimerin-domain containing protein 2; putative emu2; EMID2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human EMID2. No significant cross-reactivity or interference between Human EMID2 and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate EMID2 in samples. An antibody specific for EMID2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEMID2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EMID2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EMID2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 514074416
• NCBI GeneID: 136227
• NCBI Accession #: NP_001265492.1
• NCBI GenBank Nucleotide #: NM_001278563.2
• UniProt Accession #: Q96A83; Q32M90
• Molecular Weight: 45,131 Da
• NCBI Official Full Name: collagen alpha-1(XXVI) chain isoform 1
• NCBI Official Synonym Full Names: collagen, type XXVI, alpha 1
• NCBI Official Symbol: COL26A1
• NCBI Official Synonym Symbols: EMI6; EMU2; SH2B; EMID2
• NCBI Protein Information: collagen alpha-1(XXVI) chain; collagen alpha-1(XXVI) chain; EMI domain containing 2; emilin and multimerin domain-containing protein 2
• UniProt Protein Name: Collagen alpha-1(XXVI) chain
• UniProt Gene Name: COL26A1
• UniProt Synonym Gene Names: EMID2; EMU2; Emu2
• UniProt Entry Name: COQA1_HUMAN

NCBI Description

This gene encodes a protein containing an emilin domain and two collagen stretches. This gene may be associated with aspirin-intolerant asthma. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

EMID2: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; Golgi apparatus; proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; plasma membrane; extracellular region

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis