Typical Testing Data/Standard Curve (for reference only)
Human COL14 ELISA Kit | COL14 elisa kit
Human COL14 (Collagen Type XIV) ELISA Kit
Gene Names
COL14A1; COL14
Reactivity
Human
Synonyms
COL14; Human COL14 (Collagen Type XIV) ELISA Kit; COL14 elisa kit
Reactivity
Human
Specificity
This kit recognizes natural and some recombinant Human COL14. No significant crossreactivity or interference between Human COL14 and analogues was observed.
Sequence Length
1888
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for COL14 elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human COL14 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human COL14. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human COL14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human COL14, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human COL14. The concentration of Human COL14 in samples can be calculated by comparing the OD of the samples with the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human COL14. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human COL14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human COL14, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human COL14. The concentration of Human COL14 in samples can be calculated by comparing the OD of the samples with the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
202,668 Da
NCBI Official Full Name
collagen alpha-1(XIV) chain
NCBI Official Synonym Full Names
collagen, type XIV, alpha 1
NCBI Official Symbol
COL14A1
NCBI Official Synonym Symbols
COL14
NCBI Protein Information
collagen alpha-1(XIV) chain; collagen alpha-1(XIV) chain; alpha1 (XIV) collagen; collagen XIV; collagen type XIV; collagen type XIV/undulin; collagen, type XIV, alpha 1 (undulin)
UniProt Protein Name
Collagen alpha-1(XIV) chain
Protein Family
UniProt Gene Name
COL14A1
UniProt Entry Name
COEA1_CHICK
Uniprot Description
An adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors.
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Product Notes
The Human COL14 col14a1 (Catalog #AAA2500455) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2500455 ELISA Kit recognizes Human COL14. It is sometimes possible for the material contained within the vial of "COL14, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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