Human clusterin, CLU ELISA Kit | CLU elisa kit

Human clusterin, CLU ELISA Kit

Cat. Num. AAA165168

• Reactivity: Human
• Synonyms: clusterin; CLU; Human clusterin; CLU ELISA Kit; CLU elisa kit

• Reactivity: Human
• Sequence Length: 449

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.5ng/ml - 300ng/ml
• Sensitivity: 0.31ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLU elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Clusterin (also known as CLU) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CLU antibody. CLU present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CLU Antibody is added and binds to CLU in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CLU antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CLU. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 1048721
• NCBI GeneID: 100034172
• Molecular Weight: 52,154 Da
• NCBI Official Full Name: clusterin
• NCBI Official Synonym Full Names: clusterin
• NCBI Official Symbol: CLU
• NCBI Protein Information: clusterin
• UniProt Protein Name: Clusterin
• Protein Family: Clusterin
• UniProt Gene Name: CLU

NCBI Description

This gene encodes a protein that is similar to the human clusterin protein, a secreted chaperone that can also be found in the cell cytosol under certain stress conditions. The human protein has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. [provided by RefSeq, May 2011]

Uniprot Description

Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation ().