Human CLEC1B ELISA Kit | CLEC1B elisa kit

Human CLEC1B PicoKine ELISA Kit

Cat. Num. AAA1752601

• Gene Names: CLEC1B; CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
• Reactivity: Human
• Synonyms: CLEC1B; Human CLEC1B PicoKine ELISA Kit; Human CLEC1B; C-type lectin domain family 1 member B; C-type lectin-like receptor 2; CLEC-2; CLEC2; UNQ721; PRO1384; CLEC1B elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CLEC1B
• Sequence Length: 196

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLEC1B elisa kit

Principle of the Assay: The Picokine™ Human CLEC1B Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CLEC1B with a 96-well strip plate that is pre-coated with antibody specific for CLEC1B. The detection antibody is a biotinylated antibody specific for CLEC1B. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human CLEC1B with immunogen: Expression system for standard: NSO, Immunogen sequence: S55-P229. The kit is analytically validated with ready to use reagents. To measure Human CLEC1B, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CLEC1B in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CLEC1B in the sample.!!Background/Introduction: C-type lectin domain family 1 member B is a protein that in humans is encoded by the CLEC1B gene. This gene is mapped to 12p13.31-p13.2. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells.

NCBI and Uniprot Product Information

• NCBI GI #: 153252048
• NCBI GeneID: 51266
• NCBI Accession #: NP_001092901.1
• NCBI GenBank Nucleotide #: NP_001092901.1
• UniProt Accession #: Q9P126
• NCBI Official Full Name: C-type lectin domain family 1 member B isoform b
• NCBI Official Synonym Full Names: C-type lectin domain family 1 member B
• NCBI Official Symbol: CLEC1B
• NCBI Official Synonym Symbols: CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
• NCBI Protein Information: C-type lectin domain family 1 member B
• UniProt Protein Name: C-type lectin domain family 1 member B
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC1B
• UniProt Synonym Gene Names: CLEC2; CLEC-2
• UniProt Entry Name: CLC1B_HUMAN

NCBI Description

Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 (KLRD1; MIM 602894) and NKG2D (KLRC4; MIM 602893), that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells (Colonna et al., 2000 [PubMed 10671229]).[supplied by OMIM, Jan 2011]

Uniprot Description

CLEC1B: Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. Acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: cell surface receptor linked signal transduction; defense response; platelet activation