Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Chymotrypsinogen B2 (CTRB2) Standard Curve.)
Human Chymotrypsinogen B2 (CTRB2) ELISA Kit | CTRB2 elisa kit
Human Chymotrypsinogen B2 (CTRB2) ELISA Kit
Reactivity
Human
Synonyms
Chymotrypsinogen B2 (CTRB2); Human Chymotrypsinogen B2 (CTRB2) ELISA Kit; CTRB2; Chymotrypsin B2; CTRB2 elisa kit
Reactivity
Human
Specificity
Human Chymotrypsinogen B2 (CTRB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human CTRB2. No significant cross-reactivity or interference between Human CTRB2 and analogues was observed.
Sequence Length
263
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Chymotrypsinogen B2 (CTRB2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Chymotrypsinogen B2 (CTRB2) Standard Curve.)
Related Product Information for CTRB2 elisa kit
Description: This Human Chymotrypsinogen B2 (CTRB2) ELISA Kit employs a two-site sandwich ELISA to quantitate CTRB2 in samples. An antibody specific for CTRB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTRB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTRB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTRB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Chymotrypsin is a digestive enzyme that can perform proteolysis. Chymotrypsin preferentially cleaves peptide amide bonds where the carboxyl side of amide bond (the S1 position) is a tyrosine, tryptophan, or phenylalanine. These amino acids contain an aromatic ring in their sidechain that fits into a 'hydrophobic pocket' (the P1 position) of the enzyme. The hydrophobic and shape complementarity between the peptide substrate S1 sidechain and the enzyme P1 binding cavity accounts for the substrate specificity of this enzyme. Chymotrypsin also hydrolyzes other amide bonds in peptides at slower rates, particularly those containing leucine at the S1 position.Chymotrypsin is synthesized in the pancreas by protein biosynthesis as a precursor called chymotrypsinogen that is enzymatically inactive.
Background: Chymotrypsin is a digestive enzyme that can perform proteolysis. Chymotrypsin preferentially cleaves peptide amide bonds where the carboxyl side of amide bond (the S1 position) is a tyrosine, tryptophan, or phenylalanine. These amino acids contain an aromatic ring in their sidechain that fits into a 'hydrophobic pocket' (the P1 position) of the enzyme. The hydrophobic and shape complementarity between the peptide substrate S1 sidechain and the enzyme P1 binding cavity accounts for the substrate specificity of this enzyme. Chymotrypsin also hydrolyzes other amide bonds in peptides at slower rates, particularly those containing leucine at the S1 position.Chymotrypsin is synthesized in the pancreas by protein biosynthesis as a precursor called chymotrypsinogen that is enzymatically inactive.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
chymotrypsinogen B2
NCBI Official Synonym Full Names
chymotrypsinogen B2
NCBI Official Symbol
CTRB2
NCBI Protein Information
chymotrypsinogen B2
UniProt Protein Name
Chymotrypsinogen B2
Protein Family
UniProt Gene Name
CTRB2
Research Articles on CTRB2
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Product Notes
The Human CTRB2 ctrb2 (Catalog #AAA9715930) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715930 ELISA Kit recognizes Human CTRB2. It is sometimes possible for the material contained within the vial of "Chymotrypsinogen B2 (CTRB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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