Human Chromodomain helicase DNA binding protein 2 (CHD2) ELISA Kit | CHD2 elisa kit

Human Chromodomain helicase DNA binding protein 2 (CHD2) ELISA Kit

Cat. Num. AAA7220027

• Gene Names: CHD2; EEOC
• Reactivity: Human
• Synonyms: Chromodomain helicase DNA binding protein 2 (CHD2); Human Chromodomain helicase DNA binding protein 2 (CHD2) ELISA Kit; CHD2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHD2. No significant cross-reactivity or interference between CHD2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CHD2 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHD2 elisa kit

Intended Uses: This CHD2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCHD2. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CHD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CHD2 antibody and an CHD2-HRP conjugate. The assay sample and buffer are incubated together with CHD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHD2 concentration since CHD2 from samples and CHD2-HRP conjugate compete for the anti-CHD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CHD2 from the sample, fewer sites are left to bind CHD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHD2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CHD2 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 21594300
• NCBI GeneID: 1106
• Molecular Weight: 56,826 Da
• NCBI Official Full Name: Chromodomain helicase DNA binding protein 2
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 2
• NCBI Official Symbol: CHD2
• NCBI Official Synonym Symbols: EEOC
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 2; CHD-2; ATP-dependent helicase CHD2
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 2
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: CHD2
• UniProt Synonym Gene Names: CHD-2
• UniProt Entry Name: CHD2_HUMAN

NCBI Description

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CHD-2: Sequence-selective DNA-binding protein. Belongs to the SNF2/RAD54 helicase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; Helicase; DNA-binding

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus; nucleus

Molecular Function: ATP-dependent DNA helicase activity; DNA binding; histone binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; muscle development; transcription, DNA-dependent; chromatin modification; response to DNA damage stimulus; DNA duplex unwinding

Disease: Epileptic Encephalopathy, Childhood-onset