Human Chromodomain Y like protein (CDYL) ELISA Kit | CDYL elisa kit

Human Chromodomain Y like protein (CDYL) ELISA Kit

Cat. Num. AAA7229563

• Gene Names: CDYL; CDYL1
• Reactivity: Human
• Synonyms: Chromodomain Y like protein (CDYL); Human Chromodomain Y like protein (CDYL) ELISA Kit; CDYL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CDYL. No significant cross-reactivity or interference between CDYL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDYL and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDYL elisa kit

Intended Uses: This CDYL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDYL. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CDYL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDYL antibody and an CDYL-HRP conjugate. The assay sample and buffer are incubated together with CDYL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDYL concentration since CDYL from samples and CDYL-HRP conjugate compete for the anti-CDYL antibody binding site. Since the number of sites is limited, as more sites are occupied by CDYL from the sample, fewer sites are left to bind CDYL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDYL concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CDYL elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 221307494
• NCBI GeneID: 9425
• NCBI Accession #: NP_004815.3
• NCBI GenBank Nucleotide #: NM_004824.3
• Molecular Weight: 45,135 Da
• NCBI Official Full Name: chromodomain Y-like protein isoform a
• NCBI Official Synonym Full Names: chromodomain protein, Y-like
• NCBI Official Symbol: CDYL
• NCBI Official Synonym Symbols: CDYL1
• NCBI Protein Information: chromodomain Y-like protein; CDY-like, autosomal; testis-specific chromodomain Y-like protein
• UniProt Protein Name: Chromodomain Y-like protein
• Protein Family: Chromodomain Y-like protein
• UniProt Gene Name: CDYL
• UniProt Synonym Gene Names: CDYL1; CDY-like
• UniProt Entry Name: CDYL1_HUMAN

NCBI Description

Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CDYL: Acts as repressor of transcription. Has histone acetyltransferase activity, with a preference for histone H4. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 6p25.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; histone acetyltransferase activity; transcription corepressor activity; methylated histone residue binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; spermatogenesis; histone acetylation