Typical Testing Data/Standard Curve (for reference only)
Human Chorion specific transcription factor GCMa (GCM1) ELISA Kit | GCM1 elisa kit
Human Chorion specific transcription factor GCMa (GCM1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GCDH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GCDH antibody and an GCDH-HRP conjugate. The assay sample and buffer are incubated together with GCDH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCDH concentration since GCDH from samples and GCDH-HRP conjugate compete for the anti-GCDH antibody binding site. Since the number of sites is limited, as more sites are occupied by GCDH from the sample, fewer sites are left to bind GCDH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCDH concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a DNA-binding protein with a gcm-motif (glial cell missing motif). The encoded protein is a homolog of the Drosophila glial cells missing gene (gcm). This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins. The N-terminal DNA-binding domain confers the unique DNA-binding activity of this protein. [provided by RefSeq, Jul 2008]
Uniprot Description
GCM1: Transcription factor that is necessary for placental development. Binds to the trophoblast-specific element 2 (TSE2) of the aromatase gene enhancer.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 6p12.1
Cellular Component: transcription factor complex; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; histone deacetylase binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; positive regulation of syncytium formation by plasma membrane fusion; anatomical structure morphogenesis; regulation of transcription, DNA-dependent; astrocyte fate commitment; positive regulation of transcription from RNA polymerase II promoter
Research Articles on GCM1
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Product Notes
The Human GCM1 gcm1 (Catalog #AAA7221532) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7221532 ELISA Kit recognizes Human GCM1. It is sometimes possible for the material contained within the vial of "Chorion specific transcription factor GCMa (GCM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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