Rabbit anti-Human, Mouse ADK Polyclonal Antibody | anti-ADK antibody

ADK, NT (Adenosine Kinase, AK, Adenosine 5'-phosphotransferase) (Biotin)

Cat. Num. AAA6461600

• Gene Names: ADK; AK
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: ADK; Polyclonal Antibody; NT (Adenosine Kinase; AK; Adenosine 5'-phosphotransferase) (Biotin); anti-ADK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADK. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ADK antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 35-65 in the N-terminal region of human ADK, UniProt Accession #P55263. Species sequence homology: rat
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS6006036. This demonstrates that MBS6006036 detected the CDR2 protein (arrow).)

Product Categories/Family for anti-ADK antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 32484973
• NCBI GeneID: 132
• NCBI Accession #: NP_001114.2
• NCBI GenBank Nucleotide #: NM_001123.3
• UniProt Accession #: P55263; O00741; O00742; Q16710; Q5JQ11; Q9BTN2; B7Z783
• Molecular Weight: 40,545 Da
• NCBI Official Full Name: adenosine kinase isoform a
• NCBI Official Synonym Full Names: adenosine kinase
• NCBI Official Symbol: ADK
• NCBI Official Synonym Symbols: AK
• NCBI Protein Information: adenosine kinase; adenosine 5'-phosphotransferase
• UniProt Protein Name: Adenosine kinase
• UniProt Gene Name: ADK
• UniProt Synonym Gene Names: AK
• UniProt Entry Name: ADK_HUMAN

NCBI Description

This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

ADK: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. Defects in ADK are the cause of hypermethioninemia due to adenosine kinase deficiency (HMAKD). A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal. Belongs to the carbohydrate kinase PfkB family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.20; Nucleotide Metabolism - purine; Kinase, other

Chromosomal Location of Human Ortholog: 10q22|10q11-q24

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: metal ion binding; adenosine kinase activity; phosphotransferase activity, alcohol group as acceptor; ATP binding

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; dATP biosynthetic process; ribonucleoside monophosphate biosynthetic process; positive regulation of T cell proliferation; circadian regulation of gene expression; adenosine metabolic process; phosphorylation; purine base metabolic process; purine salvage

Disease: Hypermethioninemia Due To Adenosine Kinase Deficiency