Human Chordin Like Protein 1 (CHRDL1) ELISA Kit | CHRDL1 elisa kit

Human Chordin Like Protein 1 (CHRDL1) ELISA Kit

Cat. Num. AAA2023449

• Gene Names: CHRDL1; CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1
• Reactivity: Human
• Synonyms: Chordin Like Protein 1 (CHRDL1); Human Chordin Like Protein 1 (CHRDL1) ELISA Kit; NRLN1; CHL; VOPT; Ventroptin; Neuralin-1; Neurogenesin-1; CHRDL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHRDL1. No significant cross-reactivity or interference between CHRDL1 and analogues was observed.
• Sequence Length: 456

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.060ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CHRDL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CHRDL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRDL1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CHRDL1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CHRDL1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CHRDL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CHRDL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CHRDL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CHRDL1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 221316561
• NCBI GeneID: 91851
• NCBI Accession #: NP_001137453.1
• NCBI GenBank Nucleotide #: NM_001143981.1
• UniProt Accession #: Q9BU40; Q539E4; Q9Y3H7; B1AKD0; B4DMP3; D3DUY6; E9PGS5
• Molecular Weight: 52,026 Da
• NCBI Official Full Name: chordin-like protein 1 isoform 1
• NCBI Official Synonym Full Names: chordin-like 1
• NCBI Official Symbol: CHRDL1
• NCBI Official Synonym Symbols: CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1
• NCBI Protein Information: chordin-like protein 1
• UniProt Protein Name: Chordin-like protein 1
• Protein Family: Chordin-like protein
• UniProt Gene Name: CHRDL1
• UniProt Synonym Gene Names: NRLN1
• UniProt Entry Name: CRDL1_HUMAN

NCBI Description

This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

CHRDL1: Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development. Defects in CHRDL1 are the cause of megalocornea type 1, X-linked (MGC1). MGC1 is an eye disorder in which the corneal diameter is bilaterally enlarged (greater than 13 mm) without an increase in intraocular pressure. It may also be referred to as anterior megalophthalmos, since the entire anterior segment is larger than normal. Features of megalocornea in addition to a deep anterior chamber include astigmatic refractive errors, atrophy of the iris stroma, miosis secondary to decreased function of the dilator muscle, iridodonesis, and tremulousness, subluxation, or dislocation of the lens. Whereas most affected individuals exhibit normal ocular function, complications include cataract development and glaucoma following lenticular dislocation or subluxation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: extracellular region

Biological Process: BMP signaling pathway; neuron differentiation; ossification; eye development; compound eye development; negative regulation of BMP signaling pathway

Disease: Megalocornea