Human Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3) ELISA Kit | CHRNb3 elisa kit

Human Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3) ELISA Kit

Cat. Num. AAA454679

• Reactivity: Human
• Synonyms: Cholinergic Receptor; Nicotinic; Beta 3 (CHRNb3); Human Cholinergic Receptor; Beta 3 (CHRNb3) ELISA Kit; CHRNb3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHRNb3. No significant cross-reactivity or interference between CHRNb3 and analogues was observed.
• Sequence Length: 458

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.125ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CHRNb3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CHRNb3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRNb3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CHRNb3 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHRNb3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CHRNb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CHRNb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CHRNb3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502835
• NCBI GeneID: 1142
• NCBI Accession #: NP_000740.1
• NCBI GenBank Nucleotide #: NM_000749.4
• UniProt Accession #: Q05901; Q15827
• Molecular Weight: 52,729 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit beta-3 isoform 1
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic beta 3 subunit
• NCBI Official Symbol: CHRNB3
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-3
• UniProt Protein Name: Neuronal acetylcholine receptor subunit beta-3
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNB3

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]

Uniprot Description

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.