Human Cereblon (CRBN) ELISA Kit | CRBN elisa kit

Human Cereblon (CRBN) ELISA Kit

Cat. Num. AAA162185

• Gene Names: CRBN; MRT2; MRT2A
• Reactivity: Human
• Synonyms: Cereblon (CRBN); Human Cereblon (CRBN) ELISA Kit; CRBN elisa kit

• Reactivity: Human
• Sequence Length: 441

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.1-40ng/ml
• Sensitivity: 0.043ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRBN elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Protein cereblon (also known as CRBN) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CRBN antibody. CRBN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CRBN Antibody is added and binds to CRBN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CRBN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CRBN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 45767875
• NCBI GeneID: 51185
• UniProt Accession #: Q96SW2; Q6AI62; Q6NVZ0; Q9UHW4; B2R6H4; C9IZA9; C9JAH6
• Molecular Weight: 50,475 Da
• NCBI Official Full Name: Cereblon
• NCBI Official Synonym Full Names: cereblon
• NCBI Official Symbol: CRBN
• NCBI Official Synonym Symbols: MRT2; MRT2A
• NCBI Protein Information: protein cereblon
• UniProt Protein Name: Protein cereblon
• Protein Family: Protein cereblon
• UniProt Gene Name: CRBN

NCBI Description

This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.